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Protocol
“Hot Stuff”: The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum-Sensing Signals
Many Proteobacteria synthesize acyl-homoserine lactone (AHL) molecules for use as signals in cell density-dependent gene regulation known as quorum sensing (QS) and response. AHL detection protocols are essential...
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Chapter
Ironing Out the Biofilm Problem:The Role of Iron in Biofilm Formation
The opportunistic pathogen Pseudomonas aeruginosa causes chronic biofilm-associated infections in the lungs of cystic fibrosis patients that cannot be eradicated by antibiotics. Like most other pathogens, P. aeru...
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Article
The intracellular polyglucose storage granules of Spirochaeta aurantia
Extracts of Spirochaeta aurantia contained granules approximately 36 nm in diameter. These granules were purified by isopycnic centrifugation on CsCl gradients and shown on the basis of chemical and spectroscopic...
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Article
The basis of silver staining of bacterial lipopolysaccharides in polyacrylamide gels
When cast in a polyacrylamide gel, whole lipopolysaccharide (LPS) and the lipid A fraction of LPS fromSalmonella typhimurium andEscherichia coli O111∶B4 reacted with the silver stain described by Tsai and Frasch ...
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Article
Rifampin as a selective agent for the enumeration and isolation of spirochetes from salt marsh habitats
Spirochetes from a variety of salt marsh sediments were enumerated and isolated directly by using a prereduced complex medium containing rifampin as a selective agent. Based on cell morphology, the spirochetal...