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Article
IgE-Mediated Cannabis Allergy and Cross-Reactivity Syndromes: A Roadmap for Correct Diagnosis and Management
With increased access and decriminalization of cannabis use, cases of IgE-dependent cannabis allergy (CA) and cross-reactivity syndromes have been increasingly reported. However, the exact prevalence of cannab...
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Article
Mast Cell–Targeting Therapies in Mast Cell Activation Syndromes
Provide an overview of the expanding landscape of mast cell (MC)–targeting treatments in mast cell activation syndromes (MCAS).
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Article
Open AccessLong-term hematopoietic stem cells as a parasite niche during treatment failure in visceral leishmaniasis
Given the discontinuation of various first-line drugs for visceral leishmaniasis (VL), large-scale in vivo drug screening, establishment of a relapse model in rodents, immunophenoty**, and transcriptomics we...
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Protocol
Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques
The basis of flow cytometric allergy diagnosis is the quantification of changes in the expression of basophilic surface membrane markers (Ebo et al., Clin Exp Allergy 34: 332–339, 2004). Upon encountering spec...
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Protocol
Basophil Activation Techniques: Staining of Exteriorized Granule Matrix
The basis of traditional flow cytometry allergy diagnosis is measurement of the expression of basophilic surface activation and/or degranulation markers. Basophils, upon encounter with a specific allergen that...
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Protocol
Basophil Activation Experiments in Immediate Drug Hypersensitivity: More Than a Diagnostic Aid
Background: Correct diagnosis of immediate drug hypersensitivity reactions (IDHRs) can pose a significant challenge, mainly because of the absence of reliable in vitro tests, uncertainties associated with skin te...
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Protocol
Measurement and Functional Analysis of the Mas-Related G Protein-Coupled Receptor MRGPRX2 on Human Mast Cells and Basophils
Basophils and mast cells (MCs) are important effector cells in the immune system. For a long time, it has been known that these cells can be activated though the cross-linking of IgE antibodies bound to their ...
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Article
Letter to the Authors Concerning the Published Manuscript by Rial and Sastre: Food Allergies Caused by Allergenic Lipid Transfer Proteins: What Is Behind the Geographic Restriction?
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Article
Update on Quinolone Allergy: A Complementary Note
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Article
Open AccessIn Vitro Diagnosis of Immediate Drug Hypersensitivity Anno 2017: Potentials and Limitations
For most physicians, quantification of drug-specific immunoglobulin E (drug-sIgE) antibodies constitutes the primary in vitro measure to document immediate drug hypersensitivity reactions (IDHR). Unfortunately...
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Article
Open Access7th Drug hypersensitivity meeting: part two
Poster walk 11: miscellaneous drug hypersensitivity 2 (P92–P94, P96–P101)
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Article
Cannabis Allergy: What do We Know Anno 2015
For about a decade, IgE-mediated cannabis (marihuana) allergy seems to be on the rise. Both active and passive exposure to cannabis allergens may lead to a cannabis sensitization and/or allergy. The clinical m...
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Article
Open AccessO11 - Cor a 14: the missing link in the molecular diagnosis of hazelnut allergy?
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Protocol
Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques
The basis of flow cytometric allergy diagnosis is quantification of changes in expression of basophilic surface membrane markers (Ebo et al., Clin Exp Allergy 34: 332–339, 2004). Upon encountering specific all...
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Article
Comparison and Functional Evaluation of the Allergenicity of Different Hazelnut (Corylus avellana) Protein Extracts
This paper investigates two methodological issues of hazelnut protein extraction: the use of protease inhibitors during protein extraction and the effect of defatting hazelnuts before protein extraction. Diffe...
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Article
Hereditary Angioedema in Childhood
Hereditary angioedema (HAE) is an inherited disorder characterized by recurrent, circumscribed, nonpitting, non-pruritic, and rather painful subepithelial swelling of sudden onset, which fades during the cours...
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Chapter
Flow-Assisted Analysis of Basophils: A Valuable Instrument for In Vitro Allergy Diagnosis
It is clear that the basophil activation test (BAT) that rests upon flow-assisted quantification of in vitro-activated basophils provides the physician with a novel diagnostic tool that could rapidly spread be...
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Article
Hypersensitivity to Nadroparin Calcium
