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Article
Map** of SMV resistance gene Rsc-7 by SSR markers in soybean
Soybean mosaic virus (SMV) is one of the most prevalent pathogens that limit soybean production. In this study, segregation ratios of resistant plants to susceptible plants in P1, P2, F1, F2 populations of Kefeng...
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Article
GmZFP1 encoding a single zinc finger protein is expressed with enhancement in reproductive organs and late seed development in soybean (Glycine max)
The plant TFIIIA zinc finger proteins play important roles in plant growth and development. In this report, we isolated a single zinc finger gene, designated as GmZFP1, from soybean flowers by in silico mRNA subt...
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Article
Identification, inheritance and QTL map** of root traits related to tolerance to rhizo-spheric stresses in soybean (G. max (L.) Merr.)
A sample of soybean accessions (Glycine max (L.) Merr.) from Huanghe-Huaihe-Haihe and Middle-Lower Changjiang Valleys in China was used to identify their tolerance to rhizo-spheric stresses, including drought, al...
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Article
Genome-wide identification and characterization of putative cytochrome P450 genes in the model legume Medicago truncatula
In plants, cytochrome P450 is a group of monooxygenases existing as a gene superfamily and plays important roles in metabolizing physiologically important compounds. However, to date only a limited number of P...
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Article
Molecular cloning, expression profiling and trans-activation property studies of a DREB2-like gene from chrysanthemum (Dendranthema vestitum)
Dehydration responsive element binding (DREB) proteins are important transcription factors in plant stress response and signal transduction. In this study, a DREB homolog gene, DvDREB2A, was isolated from chrysan...
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Article
Constitutive overexpression of AOS-like gene from soybean enhanced tolerance to insect attack in transgenic tobacco
Tissue-specific and feeding-induced expression patterns of GmAOS were explored in two soybean accessions with distinct contrast in resistance grades to cotton worm: XTDD was highly susceptible and HPXQD highly re...
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Article
Cloning and functional characterization of an O-acetylserine(thiol)lyase-encoding gene in wild soybean (Glycine soja)
The terminal step of soybean cysteine synthesis is catalyzed by O-acetylserine(thiol)lyase (OAS-TL, EC 2.5.1.47). In this study, we isolated and characterized an OAS-TL gene from a wild soybean material (designat...
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Article
Characterization of O-Acetylserine(Thiol)Lyase-Encoding Genes Reveals Their Distinct but Cooperative Expression in Cysteine Synthesis of Soybean [Glycine max (L.) Merr.]
In plants, the terminal step of cysteine biosynthesis is catalyzed by O-acetylserine(thiol)lyase (OAS-TL, EC 2.5.1.47). However, to date, there is limited information on those genes involved in this pathway in so...
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Article
Identification and characterization of two chrysanthemum (Dendronthema × moriforlium) DREB genes, belonging to the AP2/EREBP family
Two DREB-like genes, designated as DmDREBa and DmDREBb, were isolated from chrysanthemum by RACE approach. The DmDREBa and DmDREBb genes encoded proteins of 191 and 185 amino acid residues with the predicted mole...
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Article
Somatic embryogenesis and plant regeneration in Chinese soybean (Glycine max (L.) Merr.)—impacts of mannitol, abscisic acid, and explant age
From a preliminary experiment on 98 Chinese soybean varieties, 12 varieties with somatic embryogenesis frequency ranging from 0.0% to 85.7% were selected for further study in order to enhance the efficiency of...
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Article
Identification and characterization of class 1 DXS gene encoding 1-deoxy-d-xylulose-5-phosphate synthase, the first committed enzyme of the MEP pathway from soybean
1-Deoxy-d-xylulose-5-phosphate synthase (DXS) catalyses the first committed step of the 2C-methyl-d-erythritol-4-phosphate (MEP) pathway, which is an alternative isoprenoids biosynthetic route that has been recen...
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Article
Detection of quantitative trait loci for phosphorus deficiency tolerance at soybean seedling stage
Phosphorus deficiency is a primary constraint to soybean productivity in acid and calcareous soils. Our aim was to map quantitative trait loci (QTL) controlling phosphorus deficiency tolerance using 152 recomb...
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Article
Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa
Gene transferability from transgenic rapeseed to various subspecies and varieties of Brassica rapa was assessed in this study. Artificial crossability was studied in 118 cultivars of 7 B. rapa subspecies and vari...
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Article
Polymorphisms of soybean isoflavone synthase and flavanone 3-hydroxylase genes are associated with soybean mosaic virus resistance
Soybean mosaic disease, caused by soybean mosaic virus (SMV), is one of the most devastating diseases that limit soybean production throughout the world. Soybean isoflavone synthase (IFS) and flavanone 3-hydro...
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Article
Map** quantitative trait loci associated with chlorophyll a fluorescence parameters in soybean (Glycine max (L.) Merr.)
Chlorophyll a fluorescence parameters can provide qualitative and quantitative information about photosynthetic processes in chloroplasts. JIP-test and modulated fluorescence (MF) parameters are commonly used chl...
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Article
Overexpression of a soybean O-acetylserine (thiol) lyase-encoding gene GmOASTL4 in tobacco increases cysteine levels and enhances tolerance to cadmium stress
O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulatio...
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Article
Proteomics-based analysis of novel genes involved in response toward soybean mosaic virus infection
Soybean mosaic virus (SMV) is one of the most serious virus diseases of soybean. However, little is known about the molecular basis of the soybean defense mechanism against this pathogen. We identified differe...
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Article
Isolation and Functional Characterization of a SERK Gene from Soybean (Glycine max (L.) Merr.)
It is well accepted that somatic embryogenesis serves a primary role in plant regeneration. However, it is also a model system to explore the regulatory and morphogenetic events in the life of a plant. To date...
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Article
Sequence and expression divergence of the AOC gene family in soybean: insights into functional diversity for stress responses
As a signaling molecule, jasmonate plays a crucial role in orchestrating plant defense responses to a variety of biotic and abiotic stresses. Allene oxide cyclase (AOC: EC5.3.99.6) catalyzes the most important...
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Article
Map** QTLs for tissue culture response in soybean (Glycine max (L.) Merr.)
Quantitative trait loci (QTLs) that control the tissue culture response in soybean were detected by using 184 recombinant inbred lines (RILs) derived from two varieties: Kefeng No.1 and Nannong 1138-2. The mol...
