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Subzonal fertilization of mouse round spermatids
Mouse round spermatids were electrofused with homologous mature oocytes to examine the behaviour of their nuclei within the ooplasm and the abilities of development. A single spermatid was injected in the peri...
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Applying bases for “double control” artificial breeding of giant panda in captivity
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Subzonal insemination with primary spermatocytes in mouse
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Article
Immunolocation of antisperm monoclonal antibody 6B10 and corresponding antigen
An antisperm monoclonal antibody 6B10 was produced by hybridoma technique of the isotype IgG. The monoclonal antibody was purified by means of ammonium sulfate precipitation and protein A-Sepharose C1-4B affin...
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Article
Antigen changes of monoclonal antibody MSH27 in process of post-testicular maturation (in mice)
An anti-mouse spermatozoon monoclonal antibody, MSH27, as well as its purified antigen, can block sperm-egg membrane fusion. As a candidate protein for sperm-egg membrane fusion, the sperm antigen was investig...
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Sperm antigen MSH27 participates in sperm-egg membrane fusion
A monoclonal antibody (mAb), MSH27 has been selected from an mAb library which has been prepared with separated mouse sperm heads as antigens. Three aspects of evidence indicated that the MSH27 antigen was inv...
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Article
The giant panda (Ailuropoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg
The giant panda skeletal muscle cells, uterus epithelial cells and mammary gland cells from an adult individual were cultured and used as nucleus donor for the construction of intenpecies embryos by transferri...
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Article
Nuclear transfer using nonquiescent adult fibroblasts from a bovine ear
The natural reproduction of mammal is sexual reproduction, which needs fertilization involving sperm and oocyte. Nuclear transfer provided an asexual reproduction method for mammal. Donor cells used in previou...
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Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda
A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blas...
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Article
Advances in interspecific pregnancy
Interspecific pregnancy in which the conceptus and female carrying the pregnancy are of different species is a key step to interspecific cloning. Cloning endangered animals by interspecific pregnancy is such a...
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Article
Effects of ubiquitin-proteasome pathway on mouse sperm capacitation, acrosome reaction and in vitro fertilization
Chlortetracycline (CTC) fluorescence patterns were used to study changes in the patterns B and AR of mouse sperm after incubation with reagents that would block the UPP. They were the monoclonal antibody again...
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Article
Serial nuclear transfer improves the development of interspecies reconstructed giant panda (Aluropoda melanoleuca) embryos
Interspecies somatic nuclear transfer (NT) may provide a new approach for preservation of the endangered rare species. Previous interspecies cloning studies have shown that a nucleus from a quiescent somatic c...
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Article
MAPK regulates cell cycle progression in pig oocytes and fertilized eggs
Oocytes collected from prepubertal gilt ovaries were matured in vitro (IVM), and fertilized in vitro (IVF) or electrically activated. Phosphorylation of mitogenactivated protein kinase (MAPK) was detected with SD...
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Article
Roles of MAP kinase signaling pathway in oocyte meiosis
Mitogen-activated protein kinase (MAPK) is a family of Ser/Thr protein kinases expressed widely in eukaryotic cells. MAPK is activated by a cascade of protein kinase phosphorylation and plays pivotal roles in ...
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Article
Embryonic stem cell as nuclear donor could promote in vitro development of the heterogeneous reconstructed embryo
The nucleus of a somatic cell could be dedifferentiated and reprogrammed in an enucleated heterogeneous oocyte. Some reconstructed oocytes could develop into blastocysts in vitro, and a few could develop into ter...
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Article
Expression of LIF, VEGF, CD57 and CD68 after the transfer of rat embryos to mouse uteri
The high failure rate of interspecific pregnancy is a major obstacle to the successful interspecific cloning of mammals. To investigate the reasons for the failure of interspecfic pregnancy between rats and mi...
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Article
Somatic cell bovine cloning: Effect of donor cell and recipients
Adult somatic cell nuclear transfer was conducted by using cultured ear fibroblast cells obtained from a Holstein female cow (GN) and a Galoway herd bull (GLV). The percentages of reconstructed eggs developed ...
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Article
Effects of different nuclear recipients on developmental potential of mouse somatic nuclear transfer embryos
In order to investigate the effects of different kinds of nuclear recipients from Kunming (KM) mouse on developmental potential of somatic nuclear transfer embryos, the enucleated M II oocytes, enucleated zygo...
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Article
Replacement of inner cell mass in mouse
The intraor inter-strain reconstituted blastocysts were produced by replacing the inner cell mass of Kunming mouse blastocysts with that of Kunming or C57BL/6 mouse strain blastocysts. A total of 192 intra-str...
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Article
Interfamily pregnancy and expression of CD57, CD68 in deciduas between golden hamster and mouse
Pregnancy between different species is one of the key steps to interspecific somatic cell cloning. Although interspecific clone embryos have been constructed, they could not develop to birth after being transf...