23 Result(s)

Article

Applying bases for “double control” artificial breeding of giant panda in captivity

Dayuan Chen, **angfen Song, Chongwen Duan, Mingwen Li… in Chinese Science Bulletin (1997)

Article

Subzonal insemination with primary spermatocytes in mouse

Ling Liu, Qingyuan Sun, Chongwen Duan, Hui Liu, **angfen Song… in Chinese Science Bulletin (1997)

Article

Sperm antigen MSH27 participates in sperm-egg membrane fusion

A monoclonal antibody (mAb), MSH27 has been selected from an mAb library which has been prepared with separated mouse sperm heads as antigens. Three aspects of evidence indicated that the MSH27 antigen was inv...

Dazhong Zhuang, **angfen Song, Guojun Hu, Qingyuan Sun… in Chinese Science Bulletin (1999)

Article

Nuclear transfer using nonquiescent adult fibroblasts from a bovine ear

The natural reproduction of mammal is sexual reproduction, which needs fertilization involving sperm and oocyte. Nuclear transfer provided an asexual reproduction method for mammal. Donor cells used in previou...

Jilong Liu, Minkang Wang, Li Lian, **song Li, Shaorong Gao… in Chinese Science Bulletin (1999)

Article

Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda

A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blas...

Bo Ding, Peng Shi, **ggong **angyu, Ya** Zhang, Dayuan Chen… in Chinese Science Bulletin (2000)

Article

Advances in interspecific pregnancy

Interspecific pregnancy in which the conceptus and female carrying the pregnancy are of different species is a key step to interspecific cloning. Cloning endangered animals by interspecific pregnancy is such a...

**chao Wang, Bojie Dai, Enkui Duan, Dayuan Chen in Chinese Science Bulletin (2001)

Article

Effects of ubiquitin-proteasome pathway on mouse sperm capacitation, acrosome reaction and in vitro fertilization

Chlortetracycline (CTC) fluorescence patterns were used to study changes in the patterns B and AR of mouse sperm after incubation with reagents that would block the UPP. They were the monoclonal antibody again...

Hongmei Wang, Changcheng Song, Chongwen Duan, Weixian Shi… in Chinese Science Bulletin (2002)

Article

Serial nuclear transfer improves the development of interspecies reconstructed giant panda (Aluropoda melanoleuca) embryos

Interspecies somatic nuclear transfer (NT) may provide a new approach for preservation of the endangered rare species. Previous interspecies cloning studies have shown that a nucleus from a quiescent somatic c...

**song Li, Dayuan Chen, Zhiming Han, Ziyu Zhu, Duancheng Wen… in Chinese Science Bulletin (2002)

Article

MAPK regulates cell cycle progression in pig oocytes and fertilized eggs

Oocytes collected from prepubertal gilt ovaries were matured in vitro (IVM), and fertilized in vitro (IVF) or electrically activated. Phosphorylation of mitogenactivated protein kinase (MAPK) was detected with SD...

Manyu Li, Hengyu Fan, Chao Tong, Dayuan Chen, Guoliang **a… in Chinese Science Bulletin (2002)

Article

Roles of MAP kinase signaling pathway in oocyte meiosis

Mitogen-activated protein kinase (MAPK) is a family of Ser/Thr protein kinases expressed widely in eukaryotic cells. MAPK is activated by a cascade of protein kinase phosphorylation and plays pivotal roles in ...

Hengyu Fan, Chao Tong, Dayuan Chen, Qingyuan Sun in Chinese Science Bulletin (2002)

Article

Embryonic stem cell as nuclear donor could promote in vitro development of the heterogeneous reconstructed embryo

The nucleus of a somatic cell could be dedifferentiated and reprogrammed in an enucleated heterogeneous oocyte. Some reconstructed oocytes could develop into blastocysts in vitro, and a few could develop into ter...

Hong Wang, Ruizhen Zheng, Ying Xu, Li Lian, Lijia An… in Chinese Science Bulletin (2002)

Article

Expression of LIF, VEGF, CD57 and CD68 after the transfer of rat embryos to mouse uteri

The high failure rate of interspecific pregnancy is a major obstacle to the successful interspecific cloning of mammals. To investigate the reasons for the failure of interspecfic pregnancy between rats and mi...

Bojie Dai, Yu**g Cao, **chao Wang, Jiaxi Zhou, Jian Zhang… in Chinese Science Bulletin (2003)

Article

Somatic cell bovine cloning: Effect of donor cell and recipients

Adult somatic cell nuclear transfer was conducted by using cultured ear fibroblast cells obtained from a Holstein female cow (GN) and a Galoway herd bull (GLV). The percentages of reconstructed eggs developed ...

Dayuan Chen, **song Li, Zhiming Han, Lei Lei, Zhonghua Liu… in Chinese Science Bulletin (2003)

Article

Effects of different nuclear recipients on developmental potential of mouse somatic nuclear transfer embryos

In order to investigate the effects of different kinds of nuclear recipients from Kunming (KM) mouse on developmental potential of somatic nuclear transfer embryos, the enucleated M II oocytes, enucleated zygo...

Lei Lei, Zhonghua Liu, Ziyu Zhu, Zhaohui Kou, Yuqi Wu, Ying Xu… in Chinese Science Bulletin (2003)

Article

Replacement of inner cell mass in mouse

The intraor inter-strain reconstituted blastocysts were produced by replacing the inner cell mass of Kunming mouse blastocysts with that of Kunming or C57BL/6 mouse strain blastocysts. A total of 192 intra-str...

Chunming Bi, Duancheng Wen, Ying Xu, Qingyuan Sun, Dayuan Chen in Chinese Science Bulletin (2003)

Article

Interfamily pregnancy and expression of CD57, CD68 in deciduas between golden hamster and mouse

Pregnancy between different species is one of the key steps to interspecific somatic cell cloning. Although interspecific clone embryos have been constructed, they could not develop to birth after being transf...

**chao Wang, Bojie Dai, Dayuan Chen, Zelong Liu, Weimin Liu… in Chinese Science Bulletin (2003)

Article

Phase-locked oscillator at 3 mm waveband using high Tc superconductor mixer mounted on pulse tube crycooler

A frequency mixing and phase locking system is designed, in which electromagnetic shielding, microwave coupling, and intermediate frequency (IF) measurement arrangements are included. In lieu of liquid nitroge...

Yafan Zhang, Weiwei Xu, Ye Dong, Dayuan Guo, Peiheng Wu in Chinese Science Bulletin (2006)