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Article
Open AccessComplete genome of Staphylococcus aureus Tager 104 provides evidence of its relation to modern systemic hospital-acquired strains
Staphylococcus aureus (S. aureus) infections range in severity due to expression of certain virulence factors encoded on mobile genetic elements (MGE). As such, characterization of the...
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Article
Rapid screening for protein solubility and expression
The Expresso® Solubility and Expression Screening System uses a simple ligase-free cloning strategy and a single PCR product to construct and test seven distinct protein fusion partners in parallel over the cours...
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Article
Open AccessFungal artificial chromosomes for mining of the fungal secondary metabolome
With thousands of fungal genomes being sequenced, each genome containing up to 70 secondary metabolite (SM) clusters 30–80 kb in size, breakthrough techniques are needed to characterize this SM wealth.
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Article
Endotoxin-free protein production—ClearColi™ technology
A new Escherichia coli strain with a genetically modified lipopolysaccharide (LPS) molecule has been created that enables protein expression without the endotoxin. Proteins expressed and purified from ClearColi™ ...
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Article
Long-span, mate-pair scaffolding and other methods for faster next-generation sequencing library creation
The NxSeq™ 40 kb Mate-Pair Cloning Kit facilitates the creation of scaffolds for de novo genome assembly. Supporting either Illumina or 454 sequencing, the kit produces long-span, mate-pair sequences with greater...