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  1. Article

    Open Access

    Complete genome of Staphylococcus aureus Tager 104 provides evidence of its relation to modern systemic hospital-acquired strains

    Staphylococcus aureus (S. aureus) infections range in severity due to expression of certain virulence factors encoded on mobile genetic elements (MGE). As such, characterization of the...

    Richard W. Davis IV, Andrew D. Brannen, Mohammad J. Hossain, Scott Monsma in BMC Genomics (2016)

  2. Article

    Rapid screening for protein solubility and expression

    The Expresso® Solubility and Expression Screening System uses a simple ligase-free cloning strategy and a single PCR product to construct and test seven distinct protein fusion partners in parallel over the cours...

    Mark Maffitt, Michele Auldridge, Saurabh Sen, Sally Floyd in Nature Methods (2015)

  3. Article

    Open Access

    Fungal artificial chromosomes for mining of the fungal secondary metabolome

    With thousands of fungal genomes being sequenced, each genome containing up to 70 secondary metabolite (SM) clusters 30–80 kb in size, breakthrough techniques are needed to characterize this SM wealth.

    ** Woo Bok, Rosa Ye, Kenneth D Clevenger, David Mead, Megan Wagner in BMC Genomics (2015)

  4. Article

    Endotoxin-free protein production—ClearColi technology

    A new Escherichia coli strain with a genetically modified lipopolysaccharide (LPS) molecule has been created that enables protein expression without the endotoxin. Proteins expressed and purified from ClearColi ...

    Uwe Mamat, Ronald W Woodard, Kathleen Wilke, Chad Souvignier, David Mead in Nature Methods (2013)

  5. Article

    Long-span, mate-pair scaffolding and other methods for faster next-generation sequencing library creation

    The NxSeq 40 kb Mate-Pair Cloning Kit facilitates the creation of scaffolds for de novo genome assembly. Supporting either Illumina or 454 sequencing, the kit produces long-span, mate-pair sequences with greater...

    Cheng-Cang Wu, Rosa Ye, Svetlana Jasinovica, Megan Wagner, Ronald Godiska in Nature Methods (2012)