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Article
Open AccessGenomic adaptations of Campylobacter jejuni to long-term human colonization
Campylobacter is a genus of bacteria that has been isolated from the gastrointestinal tract of humans and animals, and the environments they inhabit around the world. Campylobacter adapt to new environments by ch...
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Article
Open AccessThe cryo-EM structure of the bacterial flagellum cap complex suggests a molecular mechanism for filament elongation
The bacterial flagellum is a remarkable molecular motor, whose primary function in bacteria is to facilitate motility through the rotation of a filament protruding from the bacterial cell. A cap complex, consi...
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Article
Open AccessExtra Territorial Excursions by European badgers are not limited by age, sex or season
European badgers (Meles meles) in medium and high density populations show strong territorial behaviour. Territories in these populations are contiguous, well-marked and often unchanging over many years. Howev...
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Article
Open AccessBacterial periplasmic nitrate and trimethylamine-N-oxide respiration coupled to menaquinol-cytochrome c reductase (Qcr): Implications for electrogenic reduction of alternative electron acceptors
The periplasmic reduction of the electron acceptors nitrate (Em +420 mV) and trimethylamine-N-oxide (TMAO; Em +130 mV) by Nap and Tor reductases is widespread in Gram-negative bacteria and is usually considered t...
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Chapter
Indicators of Ecological Integrity
Freshwater Ecological Integrity (EI) incorporates the concepts of ecosystem “health”, unimpaired structure, composition and function and a capacity for self-renewal and, as such, it is a holistic advance over ...
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Protocol
Metabolomic Analysis of Campylobacter jejuni by Direct-Injection Electrospray Ionization Mass Spectrometry
Direct-injection mass spectrometry (DIMS) is a means of rapidly obtaining metabolomic phenotype data in both prokaryotes and eukaryotes. Given our generally poor understanding of Campylobacter metabolism, the hig...
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Article
Open AccessMetabolomic analysis of the food-borne pathogen Campylobacter jejuni: application of direct injection mass spectrometry for mutant characterisation
Campylobacter jejuni is the most frequent cause of human food-borne bacterial gastroenteritis but its physiology and biochemistry are poorly understood. Only a few amino-acids can be cat...
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Article
Evaluation of attractant flavours for use in oral vaccine baits for badgers (Meles meles)
European badgers (Meles meles) are a wildlife reservoir for Mycobacterium bovis infection (tuberculosis) in Ireland and the UK and are implicated in the transmission of infection to livestock. Vaccination of badg...
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Article
Changes in trophic linkages to shortfin eels (Anguilla australis) since the collapse of submerged macrophytes in Lake Ellesmere, New Zealand
Lake Ellesmere (Te Waihora) is a nationally important coastal brackish lake in New Zealand, however degradation in water quality and loss of submerged macrophytes over past decades have raised concerns in rega...
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Chapter
Chapter 3: Respiratory Electron Transport in Helicobacter and campylobacter
The microaerophilic, human gastro-intestinal pathogens, Campylobacter jejuni and Helicobacter pylori are closely related phylogentically, yet distinct in some major aspects of their physiology, especially with re...
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Chapter
High-Affinity C4-Dicarboxylate Uptake in Rhodobacter Capsulatus Is Mediated By A ‘Trap’ Transporter, A New Type of Periplasmic Secondary Transport System Widespread in Bacteria
Anoxygenic photosynthetic bacteria in the purple non-sulphur group have long been known to grow rapidly and with high yields on certain citric-acid cycle intermediates and their precursors, particularly pyruva...
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Protocol
Use of NMR to Study H. pylori Metabolism
Until recently, the culture of Helicobacter pylori in vitro has only been achievable with complex undefined media supplemented with blood products, such as Brain-heart infusion (BHI) medium, blood agar, chocolate...
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Chapter
Flagellate Motility, Behavioral Responses and Active Transport in Purple Non-Sulfur Bacteria
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Article
An enzyme and13C-NMR study of carbon metabolism in heliobacteria
Heliobacteria are a group of anoxygenic phototrophs that can grow photoheterotrophically in defined minimal media on only a limited range of organic substrates as carbon sources. In this study the mechanisms w...
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Article
Sequence analysis and interposon mutagenesis of a sensor-kinase (DctS) and response-regulator (DctR) controlling synthesis of the high-affinity C4-dicarboxylate transport system in Rhodobacter capsulatus
A two-component sensor-regulator system has been identified in the purple photosynthetic bacterium Rhodobacter capsulatus, which controls the expression of high-affinity C4-dicarboxylate transport activity in the...
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Article
Anaerobic degradation of trans-cinnamate and ω-phenylalkane carboxylic acids by the photosynthetic bacterium Rhodopseudomonas palustris: evidence for a β-oxidation mechanism
The mechanism responsible for the initial steps in the anaerobic degradation of trans-cinnamate and ω-phenylalkane carboxylates by the purple non-sulphur photosynthetic bacterium Rhodopseudomonas palustris was in...
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Article
Binding protein dependent transport of C4-dicarboxylates in Rhodobacter capsulatus
The characteristics of malate transport into aerobically grown cells of the purple photosynthetic bacterium Rhodobacter capsulatus were determined. A single transport system was distinguished kinetically which di...
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Chapter
Physiology and Genetics of C4-Dicarboxylate Transport in Rhodobacter capsulatus
Of those carbon sources traditionally used in studies on purple non-sulphur bacteria, the C4-dicarboxylic acids malate and succinate have long been known to be particularly effective in promoting fast growth r...
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Article
The role of auxiliary oxidants in maintaining redox balance during phototrophic growth of Rhodobacter capsulatuspropionate or butyrate
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Article
The role of auxiliary oxidants in maintaining redox balance during phototrophic growth of Rhodobacter capsulatus on propionate or butyrate
Phototrophic growth of Rhodobacter capsulatus (formerly Rhodopseudomonas capsulata) under anaerobic conditions with either butyrate or propionate as carbonsource was dependent on the presence of either CO2 or an ...