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Article
Subcortical angiopathic encephalopathy in a German kindred suggests an autosomal dominant disorder distinct from CADASIL
A cerebral arteriopathy with subcortical infarcts and leukoencephalopathy is described with a pedigree suggestive for an autosomal dominant condition. In contrast to the vasculopathy designated with the acrony...
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Article
The parental origin of new mutations in neurofibromatosis 2
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Article
Codon 178 mutation of the human prion protein gene in a German family (Backer family): sequencing data from 72-year-old celloidin-embedded brain tissue
Familial Creutzfeldt-Jakob disease was first described in a family from northern Germany in the 1920s (Backer family). PCR amplification of DNA extracted from brain tissue embedded in celloidin 72 years ago sh...
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Article
Identification of glioma-associated antigen MUC 2-63 as CD44
Monoclonal antibody MUC 2-63 recognises neurogenic tumours and has been used successfully for radioimaging human malignant gliomas. We now show that the MUC 2-63 antigen has the same tissue distribution and mo...
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Article
Sequencing data of the antiglioma antibody MUC 2-63 and strategy for construction of chimeric antibodies
The murine monoclonal antibody MUC 2-63 detects a surface antigen expressed in human gliomas as demonstrated by means of immunohistochemistry and Western blot. In addition, MUC 2-63 has been successfully used ...
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Article
Morphological, immunocytochemical and growth characteristics of three human glioblastomas established in vitro
The human glioblastoma-derived cell lines 86HG-39, 87HG-28 and 87HG-31, used for the production of monoclonal antibodies (mAbs) against glioma-associated antigens (GAA), were characterized in terms of morpholo...
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Article
Growth inhibition of experimental glioma grafts by monoclonal antibody treatment
The effects of 14AC1 monoclonal antibody (McAb) on 79FR-G-41 rat glioma cells in vitro, on the formation of metastases in lung by antibody coated glioma cells, and on the growth of glioma grafts in BALB/c-nu/n...
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Article
Monoclonal antibodies against huma gliomas
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Article
Localization of experimental glioma grafts by means of iodinated monoclonal antibodies and radionuclide imaging
Purified McAbs (14AC1) of IgG2a isotype raised against an experimental rat glioma (79 FR-G-41) were labeled with Na131I and used for in vivo imaging of glioma grafts by external body seintigraphy. Normal mouse131
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Article
Characterization of four human malignant glioma cell lines
In this paper, the characterization of four human malignant glioma cell lines is described. The four lines are positive for glial fibrillary acidic protein (GFAP) in variable amounts. One of them, LN 992, is p...
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Article
Presence and absence of virus particles in hybridomas secreting monoclonal antibodies against gliomas
Hybridoma clones were produced by fusion of splenocytes from glioma-immunized hosts and the X63-Ag8.653 mouse myeloma line and Y3-Ag.1.2.3. rat myeloma line. Oncornavirus particles were found in all clones des...
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Article
Tumor specific fluorescent and complement-dependent cytotoxic antibodies in the serum of rats with chemically induced brain gliomas
Brain tumors were induced in Sprague-Dawley and Long-Evans rats by administration of N-methyl-N-nitrosourea in the drinking water. Of these tumors, a grade 2 mixed glioma, a grade 2 to 3 astrocytoma and a grad...
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Article
Glial cell characteristics in bulk-prepared cell fractions from human brain tumours
The heterogeneity of brain tumours, especially in the glioblastoma group, makes biochemical characterization of pieces of the tumours hazardous even with extensive histological controls. This study employs a t...
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Article
Phenotypic properties of neoplastic cell lines developed from fetal rat brain cells in culture after exposure to ethylnitrosourea in vivo
We have recently reported that fetal BD IX-rat brain cells (FBC), transferred to long-term culture after a transplacental pulse of EtNU on the 18th day of gestation, undergo neoplastic transformation in vitro ...
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Article
The incidence of spontaneous tumors of the central nervous system of wistar rats
The brains of 396 old albino rats of the breed Wistar-AF/Han-EMD were examined for spontaneous tumors of the CNS and the following tumors were diagnosed: 1 oligodendroglioma, 1 astrocytoma, 1 mixed glioma, 1 p...
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Article
Gastroonkogene Wirkung von Methylnitrosoharnstoff beim Miniaturschwein
The oncogenic effect of N-methyl-N-nitrosourea was studied in Hanford miniature-pig. The compound - dissolved in 0,9% NaC1 (pH 6,1 – 6,2) and supplemented with 1% NaH2PO4 - was administered per os to the animals ...
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Article
Oncogene Wirkung von Äthylnitrosoharnstoff beim Kaninchen während der pränatalen Periode
50 mg/kg ethylnitrosourea (ENU) per dose, diluted in a 0.9%NaCl/1%NaH2PO4 solution (pH 6.2) were administered intravenously to three pregnant rabbits. The injections were carried out on the following days of gest...
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Article
Immunelektrophoretische Quantifizierung des hirnspezifischen S-100-Proteins
The quantitative determination of the brain specific protein S-100 by means of immunoelectrophoresis is described. Our immunoelectrophoretic assay uses Litex-agarose as supporting medium and rests on the measu...
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Article
Immunhistochemischer Nachweis des hirnspezifischen S-100-Proteins bei experimentellen Hirntumoren
In 50 adult Sprague-Dawley rats 42 neurogenic tumours were induced by oral application of 5 mg/kg N-methyl-N-nitrosourea through drinking water (pH 6.2). Out of this series 12 brain tumours have been set for in v...
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Article
The presence of the S-100 protein in methylnitrosourea induced tumors of the nervous system in dogs
The glia-specific protein S-100 has been demonstrated quantitatively in 1-methyl-1-nitrosourea-induced tumors of the dog CNS and PNS, which is a strong evidence for the neurooncogenic effect of MNU in the dog.
