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  1. No Access

    Chapter

    Metabolic Flux Analysis of Mammalian Cells

    Metabolic flux analysis has become a standard tool for analyzing metabolism and optimizing bioprocesses. Metabolic flux analysis makes use of a metabolic reaction network in combination with extra-cellular mea...

    D.E. Martens in Systems Biology (2007)

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    Article

    Time Dependent Responses of Glycolytic Intermediates in a Detailed Glycolytic Model of Lactococcus Lactis During Glucose Run-Out Experiments

    Glucose addition and subsequent run-out experiments were compared to simulations with a detailed glycolytic model of Lactococcus lactis. The model was constructed largely on bases of enzyme kinetic data taken fro...

    M.H.N. Hoefnagel, A. van der Burgt, D.E. Martens… in Molecular Biology Reports (2002)

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    Article

    Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers

    The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. T...

    D.E. Martens, E.A.A. Nollen, M. Hardeveld, C.A.M. van der Velden-de Groot… in Cytotechnology (1997)

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    Article

    Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers

    The death rate of Vero cells grown on Cytodex-3 microcarriers was studied as a function of the gas flow rate in a small air-lift loop reactor. The death rate may be described by first-order death-rate kinetics...

    D. E. Martens, E. A. A. Nollen, M. Hardeveld… in Cytotechnology (1996)

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    Chapter

    Physiological Aspects of Scaling Up and Monitoring

    In order to optimize the production of monoclonal antibodies several homogeneous culture systems are studied. Data are presented on cultivation of hybridoma cell line MN12 in a continuous culture system with c...

    C. A. M. van der Velden-de Groot… in Animal Cell Technology: Basic & Applied As… (1993)

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    Chapter

    To an Optimal Design of an Airlift Bioreactor for the Cultivation of Hybridomas

    One of the main problems in scaling up hybridoma cell cultures is the supply of oxygen, since sparging and agitation can lead to excessive cell death. This study concerns the relation between the air flow and ...

    D. E. Martens, Martin J. Coco, Cam van der Velden-De Groot… in From Clone to Clinic (1990)

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    Chapter

    Development of Analytical Methods for Monitoring the Stability of Antibody Formation by Hybridoma Cells in Continuous Culture Systems

    In this study an isotype specific spot-ELISA and two flow cytometric analytical procedures (FC) have been developed. Furthermore, we used different ELISA systems and isoelectric focusing (IEF) for analysis of ...

    J. M. Coco Martin, P. Koolwijk, D. E. Martens, J. W. Oberink… in From Clone to Clinic (1990)