8 Result(s)

Protocol

Measuring the Concentration of Active Alpha-1 Antitrypsin in Serum

Active Alpha-1 antitrypsin (AAT) circulates in blood in two isoforms in dynamic equilibrium: 1) native AAT, which binds irreversibly to neutrophil elastase, and 2) thiol-modified AAT, which binds reversibly to...

Cynthia L. Bristow in Alpha-1 Antitrypsin (2024)

Protocol

Lipoproteins in Negative Feedback with Alpha-1 Antitrypsin

Alpha-1 antitrypsin deficiency patients (AATD) have lower risk of myocardial infarction, a cardiovascular disease that is related to increased remnant cholesterol levels, but not to low-density lipoprotein (LD...

Cynthia L. Bristow in Alpha-1 Antitrypsin (2024)

Protocol

Purified Versus Plasma Alpha-1 Antitrypsin Effects on Cellular Activities

By adding purified exogenous alpha-1 antitrypsin (AAT) to peripheral blood mononuclear cells in 20% autologous serum and measuring AAT-induced cellular adherence, it was determined that purified AAT differs fr...

Cynthia L. Bristow in Alpha-1 Antitrypsin (2024)

Protocol

Silencing Very-Low-Density Lipoprotein Receptor Reveals Alpha-1 Antitrypsin Role in HIV Infectivity

Here we describe methods for investigating alpha-1 antitrypsin (AAT) and very-low-density lipoprotein receptor (VLDLR) interactions with infectious and non-infectious HIV-1 virions. Using silencing RNA to tran...

Cynthia L. Bristow in Alpha-1 Antitrypsin (2024)

Chapter

The Two Principal Viremias of HIV

Cynthia L. Bristow in Human Retroviral Infections (2002)