-
Article
Open AccessA Novel Method to Evaluate Ribosomal Performance in Cell-Free Protein Synthesis Systems
Cell-free protein synthesis (CFPS) systems were designed to produce proteins with a minimal set of purified components, thus offering the possibility to follow translation as well as protein folding. In order ...
-
Article
Open AccessBrightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
Life on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characte...
-
Article
Open AccessDistinct pre-initiation steps in human mitochondrial translation
Translation initiation in human mitochondria relies upon specialized mitoribosomes and initiation factors, mtIF2 and mtIF3, which have diverged from their bacterial counterparts. Here we report two distinct mi...
-
Protocol
Assembly of the Mitochondrial Translation Initiation Complex
Mitochondria maintain their own translational machinery that is responsible for the synthesis of essential components of the oxidative phosphorylation system. The mammalian mitochondrial translation system dif...
-
Article
Open AccessHuman mitochondria require mtRF1 for translation termination at non-canonical stop codons
The mitochondrial translation machinery highly diverged from its bacterial counterpart. This includes deviation from the universal genetic code, with AGA and AGG codons lacking cognate tRNAs in human mitochond...
