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Article
Open AccessUse of tryptic peptide MALDI mass spectrometry imaging to identify the spatial proteomic landscape of colorectal cancer liver metastases.
Colorectal cancer (CRC) is the second leading cause of cancer-related deaths worldwide. CRC liver metastases (CRLM) are often resistant to conventional treatments, with high rates of recurrence. Therefore, it ...
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Article
Open AccessMass spectrometry imaging spatially identifies complex-type N-glycans as putative cartilage degradation markers in human knee osteoarthritis tissue
N-Glycan alterations contribute to the pathophysiology and progression of various diseases. However, the involvement of N-glycans in knee osteoarthritis (KOA) progression at the tissue level, especially within ar...
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Article
Site-specific map** of the human SUMO proteome reveals co-modification with phosphorylation
A comprehensive analysis of the human SUMO proteome, in HeLa and U2OS cell lines and under different conditions, identifies new SUMOylated sites and reveals cross-talk between SUMO and other post-translational...
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Protocol
Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography–Tandem Mass Spectrometry
ADP-ribosylation is a posttranslational modification (PTM) that affects a variety of cellular processes. In recent years, mass spectrometry (MS)-based proteomics has become a valuable tool for studying ADP-rib...
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Article
Open AccessProteome-wide identification of the endogenous ADP-ribosylome of mammalian cells and tissue
Although protein ADP-ribosylation is involved in diverse biological processes, it has remained a challenge to identify ADP-ribose acceptor sites. Here, we present an experimental workflow for sensitive and unb...
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Article
Open AccessBiotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p
The essential vitamin biotin is a covalent and tenaciously attached prosthetic group in several carboxylases that play important roles in the regulation of energy metabolism. Here we describe increased acetyl-...
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Chapter and Conference Paper
Asymmetric Political Image Effects and the Logic of Negative Campaigning
Political marketing campaigns frequently use negative campaigning approaches, in which a candidate’s advertising criticizes an opponent, but the practice is controversial. Negative advertising in the 2012 US P...
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Article
Tousled-like kinases phosphorylate Asf1 to promote histone supply during DNA replication
During DNA replication, nucleosomes are rapidly assembled on newly synthesized DNA to restore chromatin organization. Asf1, a key histone H3-H4 chaperone required for this process, is phosphorylated by Tousled...
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Article
The pseudokinase domain of JAK2 is a dual-specificity protein kinase that negatively regulates cytokine signaling
The pseudokinase JH2 domain of JAK2 is a negative regulator of JAK2 activity, but the mechanism for this is unclear. Now it is shown that JH2 is actually an active kinase that phosphorylates negative regulator...
