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Article
Open AccessIdentification of brassinosteroid genes in Brachypodium distachyon
Brassinosteroids (BRs) are steroidal phytohormones that are involved in diverse physiological processes and affect many important traits, such as plant stature, stress tolerance, leaf angle, fertility, and gra...
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Protocol
Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness
Environmental conditions can affect stability of proteins at transcriptional or posttranscriptional levels to modulate their functions. Here we describe a method to observe changes in protein stability under d...
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Article
DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins
CRISPR-Cas9 genome editing is achieved in Arabidopsis, tobacco, lettuce and rice without introducing foreign DNA into the plants.
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Article
Analysis of anti-BZR1 antibody reveals the roles BES1 in maintaining the BZR1 levels in Arabidopsis
The transcription factor brassinazole-resistant 1 (BZR1) is a key component and positive regulator of the brassinosteroid (BR) signaling pathway. In this study, we report the cloning, expression, and purificat...
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Article
The arabidopsis gulliver2/phyB mutant exhibits reduced sensitivity to brassinazole
Brassinosteroids (BRs) are essential plant hormones with roles in normal growth and development. Based on the severe light-independent hypocotyl shortening phenotype of BR mutants, it has been proposed that BR...
