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Article
Open AccessAutomatic B cell lymphoma detection using flow cytometry data
Flow cytometry has been widely used for the diagnosis of various hematopoietic diseases. Although there have been advances in the number of biomarkers that can be analyzed simultaneously and technologies that ...
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Article
Open AccessNSMAP: A method for spliced isoforms identification and quantification from RNA-Seq
The development of techniques for sequencing the messenger RNA (RNA-Seq) enables it to study the biological mechanisms such as alternative splicing and gene expression regulation more deeply and accurately. Mo...
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Article
Open AccessIntegrative analysis of next generation sequencing for small non-coding RNAs and transcriptional regulation in Myelodysplastic Syndromes
Myelodysplastic Syndromes (MDSS) are pre-leukemic disorders with increasing incident rates worldwide, but very limited treatment options. Little is known about small regulatory RNAs and how they contribute to ...
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Article
Open AccessConditional random pattern model for copy number aberration detection
DNA copy number aberration (CNA) is very important in the pathogenesis of tumors and other diseases. For example, CNAs may result in suppression of anti-oncogenes and activation of oncogenes, which would cause...
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Article
Open AccessGenomic profiling of plasmablastic lymphoma using array comparative genomic hybridization (aCGH): revealing significant overlap** genomic lesions with diffuse large B-cell lymphoma
Plasmablastic lymphoma (PL) is a subtype of diffuse large B-cell lymphoma (DLBCL). Studies have suggested that tumors with PL morphology represent a group of neoplasms with clinopathologic characteristics corr...
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Article
Open AccessPrognostic impact of C-REL expression in diffuse large B-cell lymphoma
Diffuse large B-cell lymphoma (DLBCL) with a germinal center B-cell (GCB) phenotype is believed to confer a better prognosis than DLBCL with an activated B-cell (ABC) phenotype. Previous studies have suggested...
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Chapter
Basic Principles of Flow Cytometry
The concept of flow cytometry has been in existence for more than five decades. With the emergence of antibodies and fluorochrome chemistry and the advances in laser and computer technology, flow cytometry has...
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Article
Open AccessThe implication of identifying JAK2 V617F in myeloproliferative neoplasms and myelodysplastic syndromes with bone marrow fibrosis
The myeloproliferative neoplasms (MPN) and myelodysplastic syndromes (MDS) occasionally demonstrate overlap** morphological features including hypercellularity, mild/nonspecific dysplastic changes and variab...
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Article
Monoculture-derived T lymphocytes specific for multiple viruses expand and produce clinically relevant effects in immunocompromised individuals
Immunocompromised individuals are at high risk for life-threatening diseases, especially those caused by cytomegalovirus (CMV), Epstein-Barr virus (EBV) and adenovirus. Conventional therapeutics are primarily ...
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Article
Open AccessPrevention of an additional surgery for regional lymphadenectomy in melanoma: rapid intraoperative immunostaining of sentinel lymph node imprint smears
Sentinel lymph node (SLN) biopsy is performed at many institutions and is considered a standard of care in the management of cutaneous melanoma. The discriminatory immunostaining pattern with the 'MCW Melanoma...
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Chapter
Quantification of Tumor Load for Follicular Lymphoma by Quantifying BCL2/IGH Using Real-Time Quantitative PCR by LightCycler™
Here, we describe a newly developed real-time quantitative polymerase chain reaction (PCR) assay for detecting t(14;18)(q32;q21), the most frequent molecular lesion occurring in follicular lymphoma, using a ra...
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Article
Open AccessImproved immunohistochemical evaluation of micrometastases in sentinel lymph nodes of cutaneous melanoma with 'MCW Melanoma Cocktail' – A mixture of monoclonal antibodies to MART-1, melan-A, and tyrosinase
MART-1, Melan-A, and Tyrosinase have shown encouraging results for evaluation of melanoma micrometastases in sentinel lymph nodes, as compared to conventionally used S-100 protein and HMB-45. To achieve higher...
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Article
Open AccessColon biopsies for evaluation of acute graft-versus-host disease (A-GVHD) in allogeneic bone marrow transplant patients
Proper histomorphological interpretation of intestinal acute graft versus host disease (A-GVHD) associated with allogeneic bone marrow transplantation (BMT) is critical for clinical managaement. However, studi...
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Chapter
Measurement of MDR1 Gene Expression by Real-Time Quantitative RT-PCR Using the LightCycler Instrument
Multidrug resistance protein (P-glycoprotein) is a 170-kDa membrane glycoprotein encoded by the MDR1 gene. P-glycoprotein (P-gp) is believed to function as an ATP-dependent efflux pump for various toxins [1, 2].
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Chapter
Genoty** of Cytochrome P450 2D6*4 Mutation with Fluorescent Hybridization Probes Using LightCycler
The family of cytochrome P450 (CYP) is an important oxidative enzyme system involved in the metabolism of important drugs. More than 30 isoenzymes of CYP have been characterized in humans [1–4]. One of these, ...
