13 Result(s)
within
Christopher J Potter
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Protocol
The Q-system: A Versatile Repressible Binary Expression System
Binary expression systems are useful genetic tools for experimentally labeling or manipulating the function of defined cells. The Q-system is a repressible binary expression system that consists of a transcrip...
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Article
Open AccessInsect repellents mediate species-specific olfactory behaviours in mosquitoes
The species-specific mode of action for DEET and many other mosquito repellents is often unclear. Confusion may arise for many reasons. First, the response of a single mosquito species is often used to represe...
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Article
Open AccessOrganization of olfactory centres in the malaria mosquito Anopheles gambiae
Mosquitoes are vectors for multiple infectious human diseases and use a variety of sensory cues (olfactory, temperature, humidity and visual) to locate a human host. A comprehensive understanding of the circui...
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Protocol
The Q-System: A Versatile Expression System for Drosophila
Binary expression systems are flexible and versatile genetic tools in Drosophila. The Q-system is a recently developed repressible binary expression system that offers new possibilities for transgene expression a...
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Article
A transcriptional reporter of intracellular Ca2+ in Drosophila
The authors developed a transcriptional reporter of intracellular Ca2+ and used it to monitor activity in Drosophila sensory and neuromodulatory neurons. They demonstrate that this tool can be used to manipulate ...
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Article
Improved and expanded Q-system reagents for genetic manipulations
Binary expression systems such as the Gal4-UAS or Q-systems are useful tools for genetic manipulation in Drosophila. Here, improved reagents for the Q-system are described.
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Article
Controlling gene expression with the Q repressible binary expression system in Caenorhabditis elegans
The binary 'Q system' for controlling gene expression in Caenorhabditis elegans is reported. The system affords reversible activation of either extrachromosomal or single-copy integrated transgenes; a complementa...
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Article
Using the Q system in Drosophila melanogaster
In Drosophila, the GAL4/UAS/GAL80 repressible binary expression system is widely used to manipulate or mark tissues of interest. However, complex biological systems often require distinct transgenic manipulations...
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Article
A versatile in vivo system for directed dissection of gene expression patterns
A genetic platform allows refinement of tissue-specific expression using the upstream activating sequence–GAL4 system in Drosophila melanogaster, facilitating the segmentation of complex expression patterns and a...
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Article
Leucine-rich repeat transmembrane proteins instruct discrete dendrite targeting in an olfactory map
In Drosophila, the connections between olfactory receptor neurons and projection neurons are highly specific. Here, the authors report that two leucine-rich repeat transmembrane proteins (Capricious and Tartan) s...
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Article
Octopamine fuels fighting flies
The neural basis of aggression is poorly understood. A study in this issue used genetic scalpels to dissect the circuitry of the fly brain and identified a small cluster of octopaminergic neurons that can make...
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Article
Food for thought: a receptor finds its ligand
In C. elegans, social and solitary feeding behavior can be determined by a single amino acid change in a G protein–coupled receptor. A new study identifies ligands for this receptor and suggests how changes in be...
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Article
Akt regulates growth by directly phosphorylating Tsc2
The direct mechanism by which the serine/threonine kinase Akt (also known as protein kinase B (PKB)) regulates cell growth is unknown. Here, we report that Drosophila melanogaster Akt/PKB stimulates growth by pho...
