17 Result(s)
within
Christopher A. Makaroff
-
Article
Open AccessOverexpression of a truncated CTF7 construct leads to pleiotropic defects in reproduction and vegetative growth in Arabidopsis
Eco1/Ctf7 is essential for the establishment of sister chromatid cohesion during S phase of the cell cycle. Inactivation of Ctf7/Eco1 leads to a lethal phenotype in most organisms. Altering Eco1/Ctf7 levels or...
-
Protocol
Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana: Method 3
Immunolocalization studies to visualize the distribution of proteins on meiotic chromosomes have become an integral part of studies on meiosis in the model organism Arabidopsis thaliana. This chapter describes fi...
-
Article
The metal ion requirements of Arabidopsis thaliana Glx2-2 for catalytic activity
In an effort to better understand the structure, metal content, the nature of the metal centers, and enzyme activity of Arabidopsis thaliana Glx2-2, the enzyme was overexpressed, purified, and characterized using...
-
Article
ASK1, a SKP1 homolog, is required for nuclear reorganization, presynaptic homolog juxtaposition and the proper distribution of cohesin during meiosis in Arabidopsis
Nuclear reorganization and juxtaposition of homologous chromosomes at late leptotene/early zygotene are essential steps before chromosome synapsis at pachytene. We report the results of detailed studies, which...
-
Article
Characterization of an unusual Ds transposable element in Arabidopsis thaliana: insertion of an abortive circular transposition intermediate
The maize Ac/Dstransposable elements, which belong to the hAT transposon superfamily, are widely used as insertional mutagens in numerous plant species. Molecular studies suggest that Ac/Ds elements transpose in ...
-
Article
Characterization of an unusual Ds transposable element in Arabidopsis thaliana: Insertion of an abortive circular transposition intermediate
The maize Ac/Ds transposable elements, which belong to the hAT transposon superfamily, are widely used as insertional mutagens in numerous plant species.Molecular studies suggest that Ac/Ds elements transpose in ...
-
Article
The binding of iron and zinc to glyoxalase II occurs exclusively as di-metal centers and is unique within the metallo-β-lactamase family
Cytosolic glyoxalase 2 (GLX2-2) from Arabidopsis thaliana is a metalloenzyme that has been shown to bind a mixture of Zn, Fe, or Mn when produced in cells grown in rich media. In an effort to prepare metal-enrich...
-
Article
The dsy10 Mutation of Arabidopsis results in desynapsis and a general breakdown in meiosis
The proper pairing and recombination of chromosomes during prophase is essential for the formation of gametes during meiosis. As part of studies to identify genes required for homologous chromosome pairing and...
-
Article
Molecular characterization of glyoxalase II from Arabidopsis thaliana
Glyoxalase II is part of the glutathione-dependent glyoxalase detoxification system. In addition to its role in the detoxification of cytotoxic 2-oxo-aldehydes, specifically methylglyoxal, it has been suggeste...
-
Article
Characterization of the radish mitochondrialnad3/rps12 locus: analysis of recombination repeats and RNA editing
In order to further investigate sequences that are responsible for low-frequency recombination in plant mitochondrial DNAs and RNA editing in radish mitochondria, thenad3/rps12 locus has been isolated and charact...
-
Article
Characterization of three male-sterile mutants of Arabidopsis thaliana exhibiting alterations in meiosis
Male-sterile mutants are being studied to deepen our understanding of the complex processes of microsporogenesis and microgametogenesis. Due to difficulties associated with isolating the mutated gene, there is...
-
Article
Organ-specific reduction in the abundance of a mitochondrial protein accompanies fertility restoration in cytoplasmic male-sterile radish
The mitochondrial DNA of plants containing the male sterility-causing Ogura cytoplasm of radish contain a novel gene, orf138, that is transcribed as part of a bicistronic mRNA. Genetic studies have previously lin...
-
Article
Subunit 6 of the Fo-ATP synthase complex from cytoplasmic male-sterile radish: RNA editing and NH2-terminal protein sequencing
RNA editing and NH2-terminal processing of subunit 6 (atp6) of the mitochondrial Fo-ATPase complex has been investigated for the normal (fertile) and Ogura (male-sterile) radish cytoplasms to determine if previou...
-
Article
Characterization of the radish mitochondrial orfB locus: possible relationship with male sterility in Ogura radish
The orfB locus of the normal (fertile) and Ogura (male-sterile) radish mitochondrial genomes has been characterized in order to determine if this region, which has previously been correlated with cytoplasmic male...
-
Article
Variable intron content of the NADH dehydrogenase subunit 4 gene of plant mitochondria
The gene nad4, encoding subunit four of the mitochondrial NADH dehydrogenase complex I, has been isolated and characterized from turnip, Brassica campestris. The 8 kb turnip nad4 gene contains four exons, which p...
-
Article
The role of coxI-associated repeated sequences in plant mitochondrial DNA rearrangements and radish cytoplasmic male sterility
The gene coxI, encoding subunit I of mitochondrial cytochrome c oxidase, has been characterized from the normal (fertile) and Ogura (male-sterile) cytoplasms of radish to determine if a previously identified mito...
-
Article
Characterization of radish mitochondrial atpA: influence of nuclear background on transcription of atpA-associated sequences and relationship with male sterility
We have previously shown that the mitochondrial gene atpA, encoding the α subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm o...
