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Article
Open AccessDiscovery and characterization of genes conferring natural resistance to the antituberculosis antibiotic capreomycin
Metagenomic-based studies have predicted an extraordinary number of potential antibiotic-resistance genes (ARGs). These ARGs are hidden in various environmental bacteria and may become a latent crisis for anti...
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Article
Open AccessN-Formimidoylation/-iminoacetylation modification in aminoglycosides requires FAD-dependent and ligand-protein NOS bridge dual chemistry
Oxidized cysteine residues are highly reactive and can form functional covalent conjugates, of which the allosteric redox switch formed by the lysine-cysteine NOS bridge is an example. Here, we report a noncan...
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Article
Open AccessStructure-guided product determination of the bacterial type II diterpene synthase Tpn2
A grand challenge in terpene synthase (TS) enzymology is the ability to predict function from protein sequence. Given the limited number of characterized bacterial TSs and significant sequence diversities betw...
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Article
Open AccessStructural determination of an antibody that specifically recognizes polyethylene glycol with a terminal methoxy group
Covalent attachment of methoxy poly(ethylene) glycol (mPEG) to therapeutic molecules is widely employed to improve their systemic circulation time and therapeutic efficacy. mPEG, however, can induce anti-PEG a...
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Article
Open AccessMultiple Pleomorphic Tetramers of Thermostable Direct Hemolysin from Grimontia hollisae in Exerting Hemolysis and Membrane Binding
Oligomerization of protein into specific quaternary structures plays important biological functions, including regulation of gene expression, enzymes activity, and cell–cell interactions. Here, we report the d...
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Article
Natural separation of the acyl-CoA ligase reaction results in a non-adenylating enzyme
Acyl-coenzyme A (CoA) ligases catalyze the activation of carboxylic acids via a two-step reaction of adenylation followed by thioesterification. Here, we report the discovery of a non-adenylating acyl-CoA liga...
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Article
Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027
Comparative analysis of the enediyne biosynthetic gene clusters revealed sets of conserved genes serving as outstanding candidates for the enediyne core. Here we report the crystal structures of SgcJ and its h...
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Chapter and Conference Paper
HEp-2 Cell Images Classification Based on Textural and Statistic Features Using Self-Organizing Map
Indirect immunofluorescence (IIF) with HEp-2 cells has been used to detect antinuclear auto-antibodies (ANA) for diagnosing systemic autoimmune diseases. The aim of this study is to develop an automatic scheme...
