13 Result(s)

Chapter and Conference Paper

Map** the Genome

At the outset it is important to restate why we want to map the human genome and what such maps will tell us. There are many different types of genomic maps. A map is basically a sketch of the genome. Like a g...

Charles R. Cantor, Cassandra L. Smith in Biotechnology and the Human Genome (1988)

Article

A Streptavidin–Protein a Chimera that allows one–step production of a variety of Specific Antibody Conjugates

We have expressed a gene fusion of streptavidin and the two immunoglobulin G (IgG)–binding domains of protein A in Escherichia coli. The purified chimeric protein had full biotin–binding ability, and bound one Ig...

Takeshi Sano, Charles R. Cantor in Bio/Technology (1991)

Article

A Streptavidin Mutant Containing a Cysteine Stretch That Facilitates Production of a Variety of Specific Streptavidin Conjugates

The ability to produce specific Streptavidin conjugates has been considerably enhanced by using a Streptavidin mutant containing a cysteine stretch, in which sulfhydryl groups serve as unique conjugation sites...

Takeshi Sano, Cassandra L. Smith, Charles R. Cantor in Bio/Technology (1993)

Chapter

Can Computational Science Keep up with Evolving Technology for Genome Map** and Sequencing?

I represent a consumer of genome informatics rather than a producer in many ways. I am not an expert in informatics, and yet I am going to make occasionally what I hope are some very provocative statements abo...

Charles R. Cantor in Computational Methods in Genome Research (1994)

Article

Streptavidins with intersubunit crosslinks have enhanced stability

Natural tetrameric streptavidin has two subunit interfaces; one is a strong interface between subunits in a tightly associated dimer, and the other is a weak interface between a pair of such dimers (dimer-dime...

Gabriel O. Reznik, Sandor Vajda, Cassandra L. Smith… in Nature Biotechnology (1996)

Article

A strategy for rapid and efficient DNA sequencing by mass spectrometry

Two methods of solid-phase Sanger DNA sequencing followed by detection with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry are demonstrated. In one method, sequencing ladders gene...

Hubert Köster, Kai Tang, Dong-**g Fu, Andreas Braun… in Nature Biotechnology (1996)

Article

How will the Human Genome Project improve our quality of life?

Charles R. Cantor in Nature Biotechnology (1998)

Article

Sequencing exons 5 to 8 of the p53 gene by MALDI-TOF mass spectrometry

Matrix-assisted laser desorption ionization time of flight mass spectrometry was used to sequence exons 5 to 8 of the human p53 gene. A single tube procedure was established for target amplification and mass s...

Dong-**g Fu, Kai Tang, Andreas Braun, Dirk Reuter… in Nature Biotechnology (1998)

Chapter

The Use of MassARRAY Technology for High Throughput Genoty**

This chapter will explore the role of mass spectrometry (MS) as a detection method for genoty** applications and will illustrate how MS evolved from an expert-user-technology to a routine laboratory method i...

Christian Jurinke, Dirk van den Boom, Charles R. Cantor, Hubert Köster in Chip Technology (2002)

Article

Engineered riboregulators enable post-transcriptional control of gene expression

Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both sil...

Farren J Isaacs, Daniel J Dwyer, Chunming Ding… in Nature Biotechnology (2004)

Article

Haploty** in biomedicine—practical challenges

Charles R Cantor, Matthew R Nelson in Nature Biotechnology (2005)