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Article
Open AccessRole of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastoris
The methylotrophic yeast, Pichia pastoris, is widely used as a useful experimental tool in protein engineering and production. It is common for proteins expressed in P. pastoris to exhibit N-glycosylation. In rec...
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Article
Open AccessCrosslinking and mass spectrometry suggest that the isolated NTD domain dimer of Moloney murine leukemia virus integrase adopts a parallel arrangement in solution
Retroviral integrases (INs) catalyze the integration of viral DNA in the chromosomal DNA of the infected cell. This reaction requires the multimerization of IN to coordinate a nucleophilic attack of the 3’ end...
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Article
Production of Ultra-high Molecular Weight Poly-γ-Glutamic Acid with Bacillus licheniformis P-104 and Characterization of its Flocculation Properties
A novel strain of Bacillus licheniformis P-104 was isolated from Chinese soybean paste to produce a bioflocculant. The bioflocculant was confirmed as ultra-high molecular weight poly-γ-glutamic acid (γ-PGA) using...
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Article
Environmental assessment of heavy metal and natural radioactivity in soil around a coal-fired power plant in China
Concentrations of heavy metals and natural radionuclides in soil around a major coal-fired power plant of **’an, China were determined by using XRF and gamma ray spectrometry, respectively. The measured result...