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Article
GM crops: science, politics and communication
As the public debate in Europe about genetically modified (GM) crops heats up and the trade row between the United States and the European Union over GM food escalates, what better time to examine the issues w...
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Chapter
Plants and Human Health: Delivery of Vaccines via Transgenic Plants
One of major challenges of biotechnology is to reduce clinical innovations to economically viable practices. Plant-derived edible vaccines were first conceived and are continued to be developed with this prime...
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Article
Production of hepatitis B surface antigen in transgenic plants for oral immunization
Here we present data showing oral immunogenicity of recombinant hepatitis B surface antigen (HBsAg) in preclinical animal trials. Mice fed transgenic HBsAg potato tubers showed a primary immune response (incre...
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Chapter
Pharmaceutical Foodstuffs: Oral Immunization with Transgenic Plants
Vaccination is one of the most cost-effective medical interventions available. The public health triumphs of this century, such as the eradication of smallpox and the near eradication of polio, can be attribut...
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Article
Pharmaceutical foodstuffs—oral immunization with transgenic plants
When plants genetically engineered to produce bacterial or viral proteins are consumed as food, they trigger a protective immune response in animals and humans.
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Article
Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato
Compared with vaccine delivery by injection, oral vaccines offer the hope of more convenient immunization strategies and a more practical means of implementing universal vaccination programs throughout the wor...
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Article
Some issues for the biosafety protocol
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Article
High-tech herbal medicine: Plant-based vaccines
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Article
Generation of Transgenic Banana (Musa acuminata) Plants via Agrobacterium-Mediated Transformation
An Agrobacterium-mediated plant transformation system was developed for the generation of transgenic banana (Musa spp. van Grand Nain). This system allowed for the recovery of putative transformants within four w...
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Article
Drought resistance in rice
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Article
Sequencing and modification of psbB, the gene encoding the CP-47 protein of Photosystem II, in the cyanobacterium Synechocystis 6803
The Photosystem II protein CP-47 has been hypothesized to be involved in binding the reaction center chlorophyll. The psbB gene, encoding this protein, was cloned from the genome of the cyanobacterium Synechocyst...
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Chapter
Genetically Engineered Cytochrome B559 Mutants of the Cyanobacterium, Synechocystis 6803
Cartridge mutagenesis procedures were used to create insertion and deletion mutations in the psbE and psbF genes encoding the cytochrome b559 protein of the photosystem II complex in the cyanobacterium, Synechocy...
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Chapter
Site-Directed Mutagenesis in the Photosystem II Gene psbD, Encoding the D2 Protein
Using site-directed mutagenesis, it was shown that two histidine residues (his-197 and his-214) in the Photosystem II protein D2 play a crucial role in the function of the Photosystem II complex in the cyanoba...
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Chapter
Failure to Process the D1 Protein Inhibits the Oxidizing Side of PSII but not the Reaction Center or Reducing Side Reactions: Analysis of the LF-1 Mutant of Scenedesmus
Previous characterizations of the LF-1 mutant of Scenedesmus indicated that it is completely blocked on the oxidizing side of PSII but that the reaction center and the reducing side are relatively unaffected (...
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Chapter
Selective Thylakoid Protein Damage and Repair during Photoinhibition
When photosynthetic organisms are exposed to photon flux densities which are considerably higher than those at which the organism developed, the excessive light load results in damage to the photosynthetic mem...
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Article
Chloroplast protein phosphorylation couples plastoquinone redox state to distribution of excitation energy between photosystems
In photosynthetic membranes isolated from pea leaves, the redox state of the plastoquinone pool controls both the level of phosphorylation of the chloroplast light-harvesting pigment–protein complex (LHC) and ...
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Article
Inhibition of ion accumulation in maize roots by abscisic acid
An inhibition of root growth, a decrease in the amount of potassium (as 86Rb) and phosphate (32P) accumulation by the root, and a partial depolarization of transmembrane electropotential were observed to develop ...
