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Article
Functionality of aquaporin-2 missense mutants in recessive nephrogenic diabetes insipidus
Aquaporin-2 (AQP2) missense mutants in recessive nephrogenic diabetes insipidus (NDI) are all retained in the endoplasmic reticulum (ER), but some could function as water channels. No conclusions could be draw...
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Article
Culturing induced expression of basolateral Na+-K+-2Cl− cotransporter BSC2 in proximal tubule, aortic endothelium, and vascular smooth muscle
So far, two isoforms of the neutral Na+-K+-2Cl− cotransporter have been cloned in mammals. One isoform, BSC1, mediates apical ion entry in the renal thick ascending limb of Henle and a second, BSC2, appears to be...
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Article
Protection against hypoxic injury of rat proximal tubules by felodipine via a calcium-independent mechanism
Most evidence for a key role of calcium entry in hypoxia-induced renal damage stems from studies with calcium channel blockers. In proximal tubules, a primary site of renal ischaemic injury, only phenylalkylam...
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Article
Cellular acidification occurs during anoxia in cultured, but not in freshly isolated, rabbit proximal tubular cells
In a variety of cells it has been shown that acidosis is protective against anoxic injury. We have demonstrated previously that proximal tubule (PT) cells in primary culture were more resistant to anoxiainduce...
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Article
Effects of substrate-free anoxia and veratridine on intracellular calcium concentration in isolated rat ventricular cardiomyocytes
Cytosolic free Ca2+ concentration ([Ca2+]i) was measured in freshly isolated rat ventricular cardiomyocytes during substrate-free anoxia. Cardiomyocytes were loaded with fura-2 and incubated in an anoxic chamber ...
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Article
The effect of L-type Ca2+ channel blockers on anoxia-induced increases in intracellular Ca2+ concentration in rabbit proximal tubule cells in primary culture
Ca2+ channel blockers (CCB) have been shown to be protective against ischaemic damage of the kidney, suggesting an important role for intracellular Ca2+ ([Ca2+]i) in generating cell damage. To delineate the mecha...
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Article
The colon carcinoma cell line Caco-2 contains an H+/K+-ATPase that contributes to intracellular pH regulation
The presence of an H+/K+-ATPase and its contribution to the regulation of intracellular pH (pHi) was investigated in Caco-2 cells. The H+/K+-ATPase was detected immunologically using the monoclonal antibody 5-B6,...
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Article
Role of Na+/Ca2+ exchange in transcellular Ca2+ transport across primary cultures of rabbit kidney collecting system
Cells from connecting tubule and cortical collecting duct of rabbit kidney were isolated by immunodissection with mAb R2G9 and cultured on permeable filters. Confluent monolayers developed an amiloride-sensiti...
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Chapter and Conference Paper
Mechanisms and Sites of Transepithelial Ca2+ Transport in Kidney Cells
In epithelial cells, possible mechanisms involved in active transcellular Ca2+ transport are: a passive entry step at the apical membrane, diffusion through the cytoplasm, and active extrusion mechanisms located ...
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Article
Abstracts of papers toxicological meeting
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Article
Increased plasma calcitonin levels in young spontaneously hypertensive rats: role in disturbed phosphate homeostasis
In young spontaneously hypertensive rats (SHR) and in normotensive Wistar-Kyoto controls (WKY) several parameters of phosphate and calcium homeostasis were determined. At 6 and 8 weeks, blood analysis revealed...
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Article
Osmotic water permeabilities of brush border and basolateral membrane vesicles from rat renal cortex and small intestine
The osmotic water permeabilityP f of brush border (BBM) and basolateral (BLM) membrane vesicles from rat small intestine and renal cortex was studied by means of stopped-flow spectrophotometry. Scattered light in...
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Article
Membrane vesicles of cellular dimensions fit in two geometric series
Preparations of basal-lateral plasma membranes from rat intestinal epithelial cells were analyzed with the analytical centrifuge. In these preparations a number of well-defined membrane fractions were observed...
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Chapter
Disturbed Phosphate Metabolism in Spontaneously Hypertensive Rats
In spontaneously hypertensive rats (SHR) Ca2+ metabolism is abnormal when compared to normotensive Wistar-Kyoto rats (WKY). Disturbances in Ca2+ metabolism include a slight decrease in serum ionized Ca2+ concentr...
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Article
Kinetic properties of the ATP-dependent Ca2+ pump and the Na+/Ca2+ exchange system in basolateral membranes from rat kidney cortex
Basolateral plasma membranes from rat kidney cortex have been purified 40-fold by a combination of differential centrifugation, centrifugation in a discontinuous sucrose gradient followed by centrifugation in ...
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Article
Effect of amiloride on electrolyte transport parameters of the main duct of the rabbit mandibular salivary gland
We have studied the response of the rabbit mandibular main duct perfused in vitro to luminally administered amiloride. The half-maximal inhibitory concentrations (KI) when the duct was bathed in Cl solutions were...
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Chapter and Conference Paper
Mechanism of Active Calcium Transport in Basolateral Plasma Membranes of Rat Small Intestinal Epithelium
In addition to its role in bone formation, calcium ions play a key role in the regulation of many cellular processes. As a consequence the ionized Ca2+ concentrations of both intracellular and extracellular fluid...
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Article
Ca-stimulated ATPase in brush border and basolateral membranes of rat duodenum with high affinity sites for Ca ions
THE small intestine plays an important part in the calcium homeostasis of the body, but the mechanism by which calcium is absorbed is poorly understood. In young rats, active calcium transport occurs primarily...
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Article
Electrical resistance of rabbit submaxillary main duct: A tight epithelium with leaky cell membranes
The electrical resistance of rabbit salivary main duct epithelium has been measured. A small axial electrode, which passed current and measured potential simultaneously, was placed inside the ductal lumen. A c...
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Article
High conductance in an epithelial membrane not due to extracellular shunting
THERE is conclusive evidence for a cellular and extracellular route for ions across epithelial tissues1. The extracellular pathway is constituted by the tight junctions and the lateral intercellular space. Epithe...
