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Article
Immuno-electron microscopy reveals that the excitotoxin quinolinate is associated with the plasma membrane in human peripheral blood monocytes/macrophages
Quinolinate (QUIN), a tryptophan-derived excitotoxin, was localized ultrastructurally in human peripheral blood monocytes/macrophages (MØ) by immuno-electron microscopy. A combined carbodiimide/glutaraldehyde/...
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Article
Purification of non-infectious ganglioside preparations from scrapie-infected brain tissue
The extraction and purification of gangliosides from brains of animals infected with the scrapie agent was evaluated by scaling-down a large-scale procedure currently used with bovine brains. Inactivation expe...
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Article
Neuronal autophagic vacuoles in experimental scrapie and Creutzfeldt-Jakob disease
We report the presence of autophagic vacuoles (AV) in neuronal perikarya and neuronal processes of rodents with experimental scrapie and Creutzfeldt-Jakob disease. AV were composed of sequestrated cytoplasmic ...
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Article
Mechanism of the damage to myelinated axons in experimental Creutzfeldt-Jakob disease in mice: An ultrastructural study
We report the ultrastructural pathology of myelinated axons in mice infected experimentally with the Fujisaki strain of Creutzfeldt-Jakob disease (CJD). Initially the myelin sheath was separated into several c...
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Article
The phenotypic expression of different mutations in transmissible familial Creutzfeldt-Jakob disease
Cases of familial Creutzfeldt-Jakob disease (CJD) with mutations in the PRNP gene were analyzed for distinctive clinico-pathological and experimental transmission characteristics. An insert mutation within the...
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Article
Transmission of human T-cell leukemia virus type I from a patient with HTLV-I associated myelopathy/tropical spastic paraparesis and an asymptomatic carrier to rabbits
Rabbits were infected successfully with two strains of human T-cell leukemia virus type I (HTLV-I), one isolated from a Colombian patient with HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP...
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Chapter and Conference Paper
Prevalence of antibody to hantaviruses in humans and rodents in Italy. Provisional evidence of Hantaan-like virus infections in humans and Seoul-like virus infections in rodents
A serological investigation of 1583 healthy residents of several ecologic zones of central and northern Italy showed the presence of IgG antibodies to hantaviruses in 37 (2.3%) of them. No antibodies to hanta-...
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Article
Spread of Creutzfeldt-Jakob disease virus along visual pathways after intraocular inoculation
We studied the targeting of spongiform lesions within the visual pathways after intraocular injection with the Fujisaki strain of Creutzfeldt-Jakob disease (CJD) virus. The first lesions were observed 18 weeks...
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Article
Appearance of tubulovesicular structures in experimental Creutzfeldt-Jakob disease and scrapie preceeds the onset of clinical disease
We have consistently observed tubulovesicular structures in brain tissues during the terminal stages of naturally occurring and experimentally induced spongiform encephalopathies, irrespective of the host spec...
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Article
White matter ultrastructural pathology of experimental Creutzfeldt-Jakob disease in mice
Creutzfeldt-Jakob disease (CJD), previously regarded as a neurodegenerative disorder strictly of the gray matter, occasionally occurs as a panencephalopathic form which is characterized by severe white matter ...
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Article
Experimental hantavirus infection in nonhuman primates
Mild, transient proteinuria and azotemia were produced in three cynomolgus monkeys (Macaca fascicularis) and a chimpanzee (Pan troglodytes) following intravenous inoculation with Prospect Hill virus, a hantavirus...
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Article
Histochemical and X-ray microanalytical localization of aluminum in amyotrophic lateral sclerosis and parkinsonism-dementia of Guam
Histochemical staining for aluminum, using Solochrome azurine or Morin, provided a rapid, simple and reliable means of identifying areas and structures of the brain of interest for closer scrutiny by X-ray mic...
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Article
Simultaneous isolation of simian foamy virus and HTLV-III/LAV from chimpanzee lymphocytes following HTLV-III or LAV inoculation
Re-isolation of virus from HTLV-III B and LAV-infected chimpanzee also yielded a simian foamy virus. This virus, replicated in HTLV-III B and LAV-producing H 9 cells, had identical reverse transcriptase activi...
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Chapter
Virus Induced Slow Infections of the Central Nervous System
Infections of the nervous system have traditionally been associated with acute inflammatory diseases. Such acute viral infections may cause varied neurological syndromes such as encephalitis, paralytic poliomy...
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Article
Pathogenesis of experimental Hantaan virus infection in laboratory rats
Weanling Fischer rats inoculated intramuscularly with Hantaan virus (strain 76–118) developed subclinical infections characterized by transient viremia and shedding of virus in saliva, persistence of virus in ...
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Article
Molecular hybridization studies with scrapie brain nucleic acids
RNA and DNA fractions prepared from nucleic acids obtained from normal and scrapie-infected hamster brains following exhaustive treatment with nuclease were used as probes in a search for a specific scrapie nu...
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Article
Molecular hybridization studies with scrapie brain nucleic acids
Chromatography and hybridization techniques employing scrapie enriched fractions of hamster brains were employed to detect a scrapie-specific DNA molecule.125I-labeled DNA from eight different scrapie-enriched ha...
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Article
Antiviral neutralizing antibody to Hantaan virus as determined by plaque reduction technique
The 76–118 strain of Hantaan virus was titrated in E6 (Vero) cells by the plaque method using agarose overlay medium. Visible plaques, formed 10 days post-infection, were uniformly 2–3 mm in diameter. Dose-res...
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Article
Failure to detect scrapie virus in sheep at slaughter in a highly endemic region of France
A study was carried out in a sheep slaughterhouse located in a region of France where scrapie has been endemic for several decades. Neuropathological examination of 63 randomly selected lambs and adult sheep r...
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Article
Differential susceptibility and resistance of immunocompetent and immunodeficient mice to fatal Hantaan virus infection
Susceptibility and resistance to fatal Hantaan virus meningoencephalitis were studied in immunocompetent (nu/+) and congenitally T-cell deficient (nu/nu) CD-1 mice of different ages. Susceptibility of nu/+ mic...
