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Article
Effects of the Number of Graphene Layers and Graphene Diaphragm Size on High Frequency Electrostatic Speakers
Graphene, a promising carbon nanomaterial, has garnered significant attention owing to its chemical stability, exceptional mechanical properties, and remarkable electrical conductivity and is being used in var...
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Article
Open Access3D orientation field transform
Vascular structure enhancement is very useful in image processing and computer vision. The enhancement of the presence of the structures like tubular networks in given images can improve image-dependent diagno...
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Article
Preparation of carbonaceous monolith from polyacrylonitrile@lignin hybrid composite and its sensing and adsorption capability
A carbonaceous monolith material was produced from polyacrylonitrile@lignin (PAN@lig) composite hybrid; the sensing and adsorption capabilities of the material were evaluated. Because the two carbon-based prec...
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Article
A non-canonical role of ATG8 in Golgi recovery from heat stress in plants
Above-optimal growth temperatures, usually referred to as heat stress (HS), pose a challenge to organisms’ survival as they interfere with essential physiological functions and disrupt cellular organization. P...
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Article
Open AccessAccelerated remodeling of the mesophyll-bundle sheath interface in the maize C4 cycle mutant leaves
C4 photosynthesis in the maize leaf involves the exchange of organic acids between mesophyll (M) and the bundle sheath (BS) cells. The transport is mediated by plasmodesmata embedded in the suberized cell wall...
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Protocol
Correlative Light and Electron Microscopy Imaging of the Plant trans-Golgi Network
The plant trans-Golgi network (TGN) is a multifunctional organelle derived from the Golgi. It consists of tubulovesicular compartments scattered in the cytosol. They produce secretory vesicles delivering prote...
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Article
Open AccessElectron Tomography Analysis of Thylakoid Assembly and Fission in Chloroplasts of a Single-Cell C4 plant, Bienertia sinuspersici
Bienertia sinuspersici is a single-cell C4 plant species of which chlorenchyma cells have two distinct groups of chloroplasts spatially segregated in the cytoplasm. The central vacuole encloses most chloroplasts ...
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Article
Open AccessModular enzyme assembly for enhanced cascade biocatalysis and metabolic flux
Enzymatic reactions in living cells are highly dynamic but simultaneously tightly regulated. Enzyme engineers seek to construct multienzyme complexes to prevent intermediate diffusion, to improve product yield...
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Article
Open AccessDietary fatty acids promote lipid droplet diversity through seipin enrichment in an ER subdomain
Exogenous metabolites from microbial and dietary origins have profound effects on host metabolism. Here, we report that a sub-population of lipid droplets (LDs), which are conserved organelles for fat storage,...
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Protocol
Identification of Long Noncoding RNAs in the Develo** Endosperm of Maize
Maize endosperm consists of three distinct types of tissues, including the starchy endosperm (SE), the basal endosperm transfer cell layer (BETL), and the aleurone cell layer (AL). Compartmentalization of thes...
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Article
A whole-cell electron tomography model of vacuole biogenesis in Arabidopsis root cells
Plant vacuoles are dynamic organelles that play essential roles in regulating growth and development. Two distinct models of vacuole biogenesis have been proposed: separate vacuoles are formed by the fusion of...
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Article
Open AccessSpatio-temporal analysis of coding and long noncoding transcripts during maize endosperm development
The maize endosperm consists of three major compartmentalized cell types: the starchy endosperm (SE), the basal endosperm transfer cell layer (BETL), and the aleurone cell layer (AL). Differential genetic prog...
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Protocol
Semiautomatic Segmentation of Plant Golgi Stacks in Electron Tomograms Using 3dmod
Electron tomography is a powerful tool for visualizing subcellular organelles. With the advances in cryo-fixation techniques, it is now possible to reconstruct complex structures in cells preserved close to th...
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Protocol
3D Printing of Plant Golgi Stacks from Their Electron Tomographic Models
Three-dimensional (3D) printing is an effective tool for preparing tangible 3D models from computer visualizations to assist in scientific research and education. With the recent popularization of 3D printing ...
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Article
Open AccessKinetics and specificity of paternal mitochondrial elimination in Caenorhabditis elegans
In most eukaryotes, mitochondria are inherited maternally. The autophagy process is critical for paternal mitochondrial elimination (PME) in Caenorhabditis elegans, but how paternal mitochondria, but not maternal...
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Article
Open AccessArabidopsis protein disulfide isomerase-8 is a type I endoplasmic reticulum transmembrane protein with thiol-disulfide oxidase activity
In eukaryotes, classical protein disulfide isomerases (PDIs) facilitate the oxidative folding of nascent secretory proteins in the endoplasmic reticulum by catalyzing the formation, breakage, and rearrangement...
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Article
ZrTiO4 porous ceramics fabricated from particle-stabilized wet foam by direct foaming
Indent ZrTiO4 ceramics are widely used to manufacture electrical and optical devices. This study reveals an approach for the production of micro-porous ceramics consisting of TiO2 and ZrO2 by using a direct foami...
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Protocol
STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells
Because of the weak penetrating power of electrons, the signal-to-noise ratio of a transmission electron micrograph (TEM) worsens as section thickness increases. This problem is alleviated by the use of the sc...
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Protocol
Unconventional Protein Secretion in Plants
Unconventional protein secretion (UPS) describes secretion pathways that bypass one or several of the canonical secretion pit-stops on the way to the plasma membrane, and/or involve the secretion of leaderless...
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Article
Characterization of a Chlamydomonas reinhardtii mutant defective in a maltose transporter
Microalgae are potential sources of energy and high-value materials. To decipher the process of energy metabolism in green algae, we created a mutant pool of strain CC-503 of the model green microalga Chlamydomon...
