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Article
Open AccessAn insight into the sialotranscriptome and virome of Amazonian anophelines
Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and anti-inflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of e...
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Article
Open AccessDeciphering the olfactory repertoire of the tiger mosquito Aedes albopictus
The Asian tiger mosquito Aedes albopictus is a highly invasive species and competent vector of several arboviruses (e.g. dengue, chikungunya, Zika) and parasites (e.g. dirofilaria) of public health importance. Co...
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Article
Open AccessAnopheline salivary protein genes and gene families: an evolutionary overview after the whole genome sequence of sixteen Anopheles species
Mosquito saliva is a complex cocktail whose pharmacological properties play an essential role in blood feeding by counteracting host physiological response to tissue injury. Moreover, vector borne pathogens ar...
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Article
Open AccessGenomic organization and splicing evolution of the doublesex gene, a Drosophila regulator of sexual differentiation, in the dengue and yellow fever mosquito Aedes aegypti
In the model system Drosophila melanogaster, doublesex (dsx) is the double-switch gene at the bottom of the somatic sex determination cascade that determines the differentiation of sexually dimorphic traits. Homo...
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Article
Open AccessAn annotated catalogue of salivary gland transcripts in the adult female mosquito, Ædes ægypti*
Saliva of blood-sucking arthropods contains a cocktail of antihemostatic agents and immunomodulators that help blood feeding. Mosquitoes additionally feed on sugar meals and have specialized regions of their g...
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Article
Distribution of the Transposable Element Minos in the Genus Drosophila
We analyzed 28 species of the genus Drosophilafor the presence of the Tc1-like transposable element Minosusing Southern blot hybridization under high stringency conditions. The Minostransposon was found in member...
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Chapter
The polymerase chain reaction (PCR) and RT-PCR
The polymerase chain reaction (PCR), a method for the in vitro enzymatic amplification of DNA, has facilitated greatly the analysis of complex genomes by permitting the characterization and cloning of DNA sequenc...