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Article
Open AccessDeletion of QDR genes in a bioethanol-producing yeast strain reduces propagation of contaminating lactic acid bacteria
Bacterial contaminations in yeast fermentation tanks are a recurring problem for the bioethanol production industry. Lactic acid bacteria (LAB), particularly of the genus Lactobacillus, are the most common contam...
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Article
Open AccessA plant plasma-membrane H+-ATPase promotes yeast TORC1 activation via its carboxy-terminal tail
The Target of Rapamycin Complex 1 (TORC1) involved in coordination of cell growth and metabolism is highly conserved among eukaryotes. Yet the signals and mechanisms controlling its activity differ among taxa,...
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Article
Open AccessUbiquitylation and endocytosis of the human LAT1/SLC7A5 amino acid transporter
The human L-type amino acid transporter 1 (LAT1), also known as SLC7A5, catalyzes the transport of large neutral amino acids across the plasma membrane. As the main transporter of several essential amino acids...
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Protocol
Measuring the Activity of Plasma Membrane and Vacuolar Transporters in Yeast
The yeast proteome includes about 300 polytopic membrane proteins known or predicted to function as transporters. Such proteins ensure active or passive transport of small ions or metabolites across the plasma...
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Article
Open AccessFTY720-induced endocytosis of yeast and human amino acid transporters is preceded by reduction of their inherent activity and TORC1 inhibition
FTY720 is a sphingoid base analog that acts as an anticancer agent in animal models. Its effect on tumor cells stems largely from its ability to trigger endocytosis of several nutrient transporters. The observ...
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Chapter
Function and Regulation of Fungal Amino Acid Transporters: Insights from Predicted Structure
Amino acids constitute a major nutritional source for probably all fungi. Studies of model species such as the yeast Saccharomyces cerevisiae and the filamentous fungus Aspergillus nidulans have shown that they p...
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Article
Open AccessThe AP-3 adaptor complex mediates sorting of yeast and mammalian PQ-loop-family basic amino acid transporters to the vacuolar/lysosomal membrane
The limiting membrane of lysosomes in animal cells and that of the vacuole in yeast include a wide variety of transporters, but little is known about how these proteins reach their destination membrane. The ma...
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Article
Open AccessNitrogen catabolite repressible GAP1 promoter, a new tool for efficient recombinant protein production in S. cerevisiae
Decades of work requiring heterologous expression of eukaryotic proteins have shown that no expression system can be considered as the panacea and the appropriate expression strategy is often protein-dependent...
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Chapter and Conference Paper
Gaussian Graphical Models to Infer Putative Genes Involved in Nitrogen Catabolite Repression in S. cerevisiae
Nitrogen is an essential nutrient for all life forms. Like most unicellular organisms, the yeast Saccharomyces cerevisiae transports and catabolizes good nitrogen sources in preference to poor ones. Nitrogen cata...
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Article
Open AccessMachine learning techniques to identify putative genes involved in nitrogen catabolite repression in the yeast Saccharomyces cerevisiae
Nitrogen is an essential nutrient for all life forms. Like most unicellular organisms, the yeast Saccharomyces cerevisiae transports and catabolizes good nitrogen sources in preference to poor ones. Nitrogen cata...
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Article
Structural involvement in substrate recognition of an essential aspartate residue conserved in Mep/Amt and Rh-type ammonium transporters
Ammonium transport proteins belonging to the Mep/Amt/Rh family are spread throughout all domains of life. A conserved aspartate residue plays a key role in the function of Escherichia coli AmtB. Here, we show tha...
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Chapter
Diversity of Nitrogen Metabolism Among Yeast Species: Regulatory and Evolutionary Aspects
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Chapter
Role of transporter-like sensors in glucose and amino acid signalling in yeast
Nutrient sensing is crucial to enabling cells to respond properly to changing food supplies. Recent studies suggest that specific members of large transporter families have no detectable transport activity and...
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Chapter
Membrane trafficking of yeast transporters: mechanisms and physiological control of downregulation
Of the 125 plasma membrane transporters thus far identified in the yeast S. cerevisiae, a growing number is reported to be subject to tight control at membrane trafficking level, in addition to control at transcr...
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Article
Functional profiling of the Saccharomyces cerevisiae genome
Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systema...
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Article
The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast
The Rhesus blood-group antigens are defined by a complex association of membrane polypeptides that includes the non-glycosylated Rh proteins (RhD and RhCE) and the RHag glycoprotein, which is strictly required...
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Article
Cloning and expression of the UGA4 gene coding for the inducible GABA-specific transport protein of Saccharomyces cerevisiae
Transport of 4-aminobutyric acid (GABA) in Saccharomyces cerevisiae is mediated by three transport systems: the general amino acid permease (GAP1 gene), the proline permease (PUT4 gene), and a specific GABA perme...
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Article
The UGA43 negative regulatory gene of Saccharomyces cerevisiae contains both a GATA-1 type zinc finger and a putative leucine zipper
The UGA43 gene of Saccharomyces cerevisiae is required for repression of inducible genes involved in the utilization of 4-aminobutyric acid (GABA) or urea as nitrogen sources. The UGA43 gene has been cloned by co...
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Article
The UGA3 gene regulating the GABA catabolic pathway in Saccharomyces cerevisiae codes for a putative zinc-finger protein acting on RNA amount
The UGA3 gene of Saccharomyces cerevisiae is required for 4-aminobutyric acid (GABA)-dependent induction of the UGA1, UGA2 and UGA4 genes which encode the two GABA catabolic enzymes and a GABA-specific permease, ...
