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Article
Simultaneous ribosome profiling of hundreds of microbes from the human microbiome
Ribosome profiling enables sequencing of ribosome-bound fragments of RNA, revealing which transcripts are being translated as well as the position of ribosomes along mRNAs. Although ribosome profiling has been...
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Article
Open AccessComparative genomics identifies thousands of candidate structured RNAs in human microbiomes
Structured RNAs play varied bioregulatory roles within microbes. To date, hundreds of candidate structured RNAs have been predicted using informatic approaches that search for motif structures in genomic seque...
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Article
Open AccessMetaRibo-Seq measures translation in microbiomes
No method exists to measure large-scale translation of genes in uncultured organisms in microbiomes. To overcome this limitation, we develop MetaRibo-Seq, a method for simultaneous ribosome profiling of tens t...