-
Article
Open AccessA transgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis
Maintenance of calcium homeostasis is important for proper endoplasmic reticulum (ER) function. When cellular stress conditions deplete the high concentration of calcium in the ER, ER-resident proteins are sec...
-
Article
Open AccessIdentification of ER/SR resident proteins as biomarkers for ER/SR calcium depletion in skeletal muscle cells
Aberrations to endoplasmic/sarcoplasmic reticulum (ER/SR) calcium concentration can result in the departure of endogenous proteins in a phenomenon termed exodosis. Redistribution of the ER/SR proteome can have...
-
Article
Open AccessThe overexpression of GDNF in nucleus accumbens suppresses alcohol-seeking behavior in group-housed C57Bl/6J female mice
Craving for alcohol, in other words powerful desire to drink after withdrawal, is an important contributor to the development and maintenance of alcoholism. Here, we studied the role of GDNF (glial cell line-d...
-
Article
Trophic activities of endoplasmic reticulum proteins CDNF and MANF
Mesencephalic astrocyte-derived neurotrophic factor (MANF) and cerebral dopamine neurotrophic factor (CDNF) are endoplasmic reticulum (ER) luminal proteins that confer trophic activities in a wide range of tis...
-
Article
Cas9 Ribonucleoprotein Complex Delivery: Methods and Applications for Neuroinflammation
The CRISPR/Cas9 system is a revolutionary gene editing technology that combines simplicity of use and efficiency of mutagenesis. As this technology progresses toward human therapies, valid concerns including o...
-
Article
Pifithrin-Alpha Reduces Methamphetamine Neurotoxicity in Cultured Dopaminergic Neurons
Methamphetamine (Meth) is a widely abused stimulant. High-dose Meth induces degeneration of dopaminergic neurons through p53-mediated apoptosis. A recent study indicated that treatment with the p53 inhibitor, ...
-
Article
Neurons Internalize Functionalized Micron-Sized Silicon Dioxide Microspheres
Microparticles have potential as neuron-specific delivery platforms and devices with many applications in neuroscience, pharmacology, and biomedicine. To date, most literature suggests that neurons are not pha...
-
Article
Long Evans rat spermatogonial lines are effective germline vectors for transgenic rat production
Long Evans rat strains are applied as research models in a broad spectrum of biomedical fields (>15,800 citations, NCBI PubMed). Here, we report an approach to genetically modify the Long Evans rat germline in...
-
Article
Open Access9-cis retinoic acid induces neurorepair in stroke brain
The purpose of this study was to examine the neurorestorative effect of delayed 9 cis retinoic acid (9cRA) treatment for stroke. Adult male rats received a 90-min right distal middle cerebral artery occlusion ...
-
Article
CYP3A5 Mediates Effects of Cocaine on Human Neocorticogenesis: Studies using an In Vitro 3D Self-Organized hPSC Model with a Single Cortex-Like Unit
Because of unavoidable confounding variables in the direct study of human subjects, it has been difficult to unravel the effects of prenatal cocaine exposure on the human fetal brain, as well as the cellular a...
-
Article
Differential modulation of methamphetamine-mediated behavioral sensitization by overexpression of Mu opioid receptors in nucleus accumbens and ventral tegmental area
Repeated administration of methamphetamine (Meth) induces behavioral sensitization which is characterized by a progressive increase in locomotor response after each injection. Previous studies have shown that ...
-
Article
Behavioral and Physiological Effects of a Novel Kappa-Opioid Receptor-Based DREADD in Rats
In the past decade, novel methods using engineered receptors have enabled researchers to manipulate neuronal activity with increased spatial and temporal specificity. One widely used chemogenetic method in mic...
-
Article
Open AccessDirect wavefront sensing for high-resolution in vivo imaging in scattering tissue
Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specim...
-
Article
Cortical activation of accumbens hyperpolarization-active NMDARs mediates aversion-resistant alcohol intake
The authors show that optogenetic inhibition of inputs from medial prefrontal cortex (mPFC) or insula to the nucleus accumbens core (NAcore) inhibits aversion-resistant alcohol intake. Alcohol-drinking rats sh...
-
Article
Open AccessHypothalamic proteoglycan syndecan-3 is a novel cocaine addiction resilience factor
Proteoglycans like syndecan-3 have complex signaling roles in addition to their function as structural components of the extracellular matrix. Here, we show that syndecan-3 in the lateral hypothalamus has an u...
-
Article
Methamphetamine activates nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and induces human immunodeficiency virus (HIV) transcription in human microglial cells
Human immunodeficiency virus (HIV) primarily infects glial cells in the central nervous system (CNS). Recent evidence suggests that HIV-infected individuals who abuse drugs such as methamphetamine (METH) have ...
-
Article
Suppression of endogenous PPARγ increases vulnerability to methamphetamine-induced injury in mouse nigrostriatal dopaminergic pathway
Methamphetamine is a commonly abused drug and dopaminergic neurotoxin. Repeated administration of high doses of methamphetamine induces programmed cell death, suppression of dopamine release, and reduction in ...
-
Article
Is GDNF beneficial in Parkinson disease?
Glial cell line-derived neurotrophic factor (GDNF) has proved efficacious in treatment of neurodegeneration in toxin-induced models of Parkinson disease (PD), but clinical trials have been equivocal. Do the re...
-
Article
Transgenic animal models of neurodegeneration based on human genetic studies
The identification of genes linked to neurodegenerative diseases such as Alzheimer’s disease (AD), amyotrophic lateral sclerosis (ALS), Huntington’s disease (HD) and Parkinson’s disease (PD) has led to the dev...
-
Article
Targeted disruption of cocaine-activated nucleus accumbens neurons prevents context-specific sensitization
Until now, no tools existed to selectively manipulate the small number of sparsely distributed neurons activated during a drug-related learned behavior. Here the authors describe a new method for selectively i...
