23 Result(s)
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Article
Open AccessVariant analysis of grain size related genes in the genus Sorghum
Grain size and weight are two of the most important determinants of crop yield. Key genes associated with the grain size and weight have been identified in major crops. However, studies on the genetic basis of...
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Article
Open AccessTranscript profiles of wild and domesticated sorghum under water-stressed conditions and the differential impact on dhurrin metabolism
Australian native species of sorghum contain negligible amounts of dhurrin in their leaves and the cyanogenesis process is regulated differently under water-stress in comparison to domesticated...
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Article
Open AccessRegulation of dhurrin pathway gene expression during Sorghum bicolor development
Developmental and organ-specific expression of genes in dhurrin biosynthesis, bio-activation, and recycling offers dynamic metabolic responses optimizing growth and defence responses in Sorghum.
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Article
Open AccessNerylneryl diphosphate is the precursor of serrulatane, viscidane and cembrane-type diterpenoids in Eremophila species
Eremophila R.Br. (Scrophulariaceae) is a diverse genus of plants with species distributed across semi-arid and arid Australia. It is an ecologically important genus that also holds cultural significance for many ...
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Article
Phytochemistry and bioactivity of Acacia sensu stricto (Fabaceae: Mimosoideae)
Acacia sensu lato (Fabaceae: Mimosoideae) was recently retypified and divided into five genera worldwide: Acacia, Acaciella, Mariosousa, Senegalia and Vachellia. Acacia sensu stricto is now considered to be a lar...
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Article
Lotus japonicus flowers are defended by a cyanogenic β-glucosidase with highly restricted expression to essential reproductive organs
Flowers and leaves of Lotus japonicus contain α-, β-, and γ-hydroxynitrile glucoside (HNG) defense compounds, which are bioactivated by β-glucosidase enzymes (BGDs). The α-HNGs are referred to as cyanogenic gluco...
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Article
Cyanogenic glycosides: a case study for evolution and application of cytochromes P450
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Chapter and Conference Paper
Biofortification of Cassava Using Molecular Breeding
Cassava, Manihot esculenta, is the most important root crop and the third most important staple food in the Tropics. Cassava has gained such a high importance because (1) cassava produces well on poor soils and m...
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Article
Cyanogenic glycosides: a case study for evolution and application of cytochromes P450
Cyanogenic glycosides are ancient biomolecules found in more than 2,650 higher plant species as well as in a few arthropod species. Cyanogenic glycosides are amino acid-derived β-glycosides of α-hydroxynitrile...
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Article
Reconstitution of cyanogenesis in barley (Hordeum vulgare L.) and its implications for resistance against the barley powdery mildew fungus
Barley (Hordeum vulgare L.) produces a leucine-derived cyanogenic β-d-glucoside, epiheterodendrin that accumulates specifically in leaf epidermis. Barley leaves are not cyanogenic, i.e. they do not possess the ab...
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Article
Photoinhibition of Photosystem I in field-grown barley (Hordeum vulgare L.): Induction, recovery and acclimation
The effects of exposure of a field-grown winter cultivar of barley (Hordeum vulgare L.) to Photosystem I (PS I) photoinhibitory conditions in the form of bright day-light combined with chilling conditions were in...
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Article
Photoinhibition of Photosystem I damages both reaction centre proteins PSI-A and PSI-B and acceptor-side located small Photosystem I polypeptides
Photoinhibition of Photosystem I at chilling temperatures was investigated. Illumination of barley and cucumber leaves at 4°C induced a lowered Photosystem I activity. In barley, the reaction centre proteins P...
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Article
Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogenic glucoside dhurrin
A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three conse...
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Chapter
Photoinhibitory Damage of Barley Photosystem I at Chilling Temperatures Induced under Controlled Illumination is also Identified in the Field
Light is needed for photosynthesis but may cause photoinhibition when energy input exceeds consumption (1, 2). Illumination at chilling temperatures further increases the photoinhibitory damage (1–3).
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Article
Import of the barley PSI-F subunit into the thylakoid lumen of isolated chloroplasts
A full-length cDNA clone encoding the PSI-F subunit of barley photosystem I has been isolated and sequenced. The open reading frame encodes a precursor polypeptide with a deduced molecular mass of 24837 Da. Th...
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Article
Precursors of one integral and five lumenal thylakoid proteins are imported by isolated pea and barley thylakoids: optimisation of in vitro assays
In vitro assays for the import of proteins by isolated pea thylakoids have been refined and optimised with respect to (a) the method of thylakoid preparation, (b) the concentration of thylakoids in the import as...
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Article
A cDNA clone from barley encoding the precursor from the photosystem I polypeptide PSI-G: Sequence similarity to PSI-K
A cDNA clone encoding the photosystem I subunit, PSI-G was isolated from barley using an oligonucleotide specifying a partial amino acid sequence from a 9 kDa polypeptide of barley photosystem I. The 724 bp se...
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Chapter
Characterization of a cDNA Clone for the PsaH Gene from Barley and mRNA Level of PS I Genes in Light-Induced Barley Seedlings
Photosystem I (PS I) catalyzes the coupled photoreduction of ferredoxin and photooxidation of plastocyanin. PS I core preparations from plants and cyanobacteria has been shown to contain a number of polypeptid...
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Chapter
Nearest Neighbour Analysis of the Photosystem I Subunits in Barley and Their Binding of Ferredoxin
Photosystem I (PS I) mediates electron transfer from plastocyanin to ferredoxin through the reaction center P700 and the five electron acceptors A0, A1, X, A and B (1). PS I preparations from barley contain the r...
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Chapter
Photosystem I in Barley: Subunit PSI-F is Not Essential for the Interaction with Plastocyanin
Photosystem I (PS I) preparations from barley contain polypeptides with apparent molecular masses of 82 (PSI-A and PSI-B), 18 (PSI-D), 16 (PSI-E), 14, 9.5 (PSI-H), 9 (PSI-C), 4, and 1.5 kDa (PSI-I) (1, 2). The...
