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Article
Open AccessA time-resolved, multi-symbol molecular recorder via sequential genome editing
DNA is naturally well suited to serve as a digital medium for in vivo molecular recording. However, contemporary DNA-based memory devices are constrained in terms of the number of distinct ‘symbols’ that can b...
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Article
Precise genomic deletions using paired prime editing
Current methods to delete genomic sequences are based on clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 and pairs of single-guide RNAs (sgRNAs), but can be inefficient and imprecise, w...
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Article
Author Correction: lentiMPRA and MPRAflow for high-throughput functional characterization of gene regulatory elements
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
A systematic evaluation of the design and context dependencies of massively parallel reporter assays
Massively parallel reporter assays (MPRAs) functionally screen thousands of sequences for regulatory activity in parallel. To date, there are limited studies that systematically compare differences in MPRA des...
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Article
lentiMPRA and MPRAflow for high-throughput functional characterization of gene regulatory elements
Massively parallel reporter assays (MPRAs) can simultaneously measure the function of thousands of candidate regulatory sequences (CRSs) in a quantitative manner. In this method, CRSs are cloned upstream of a ...
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Article
Disseminated Pneumocystis jirovecii Infection with Osteomyelitis in a Patient with CTLA-4 Haploinsufficiency
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Article
Open AccessSaturation mutagenesis of twenty disease-associated regulatory elements at single base-pair resolution
The majority of common variants associated with common diseases, as well as an unknown proportion of causal mutations for rare diseases, fall in noncoding regions of the genome. Although catalogs of noncoding ...
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Article
Accurate classification of BRCA1 variants with saturation genome editing
Variants of uncertain significance fundamentally limit the clinical utility of genetic information. The challenge they pose is epitomized by BRCA1, a tumour suppressor gene in which germline loss-of-function vari...
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Article
Multiplex assessment of protein variant abundance by massively parallel sequencing
Determining the pathogenicity of genetic variants is a critical challenge, and functional assessment is often the only option. Experimentally characterizing millions of possible missense variants in thousands ...
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Article
Learning new vocabulary in German: the effects of inferring word meanings, type of feedback, and time of test
In the present study, introductory-level German students read a simplified story and learned the meanings of new German words by reading English translations in marginal glosses versus trying to infer (i.e., g...
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Article
From industry to academia
A postdoc stint in industry can mean better pay and superior facilities. But will doing such a fellowship jeopardize your academic aspirations? Beth Martin investigates.
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Chapter and Conference Paper
Towards a Reusable SoC Design Methodology
As industry generates and adopts complex methodologies for managing and designing system-on-a-chip (SoC) products, new challenges arise for effective knowledge transfer. This paper describes the specific chall...
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Article
Ploidy of small individual embryo-like structures from maize anther cultures treated with chromosome doubling agents and calli derived from them
In order to determine the ploidy of individual embryo-like structures (ELSs) following chromosome doubling treatments, a method was developed to determine the DNA content (ploidy level) of nuclei from single E...
