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Article
Organ-on-chip models for infectious disease research
Research on microbial pathogens has traditionally relied on animal and cell culture models to mimic infection processes in the host. Over recent years, developments in microfluidics and bioengineering have led...
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Article
The hyphal-specific toxin candidalysin promotes fungal gut commensalism
The fungus Candida albicans frequently colonizes the human gastrointestinal tract, from which it can disseminate to cause systemic disease. This polymorphic species can transition between growing as single-celled...
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Article
Secretion of the fungal toxin candidalysin is dependent on conserved precursor peptide sequences
The opportunistic fungal pathogen Candida albicans damages host cells via its peptide toxin, candidalysin. Before secretion, candidalysin is embedded in a precursor protein, Ece1, which consists of a signal pepti...
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Article
Open AccessSelection of cross-reactive T cells by commensal and food-derived yeasts drives cytotoxic TH1 cell responses in Crohn’s disease
Aberrant CD4+ T cell reactivity against intestinal microorganisms is considered to drive mucosal inflammation in inflammatory bowel diseases. The disease-relevant microbial species and the corresponding microorga...
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Article
Open AccessCandida expansion in the gut of lung cancer patients associates with an ecological signature that supports growth under dysbiotic conditions
Candida species overgrowth in the human gut is considered a prerequisite for invasive candidiasis, but our understanding of gut bacteria promoting or restricting this overgrowth is still limited. By integrating c...
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Article
Author Correction: Immune regulation by fungal strain diversity in inflammatory bowel disease
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Article
Open AccessLactobacillus rhamnosus colonisation antagonizes Candida albicans by forcing metabolic adaptations that compromise pathogenicity
Intestinal microbiota dysbiosis can initiate overgrowth of commensal Candida species – a major predisposing factor for disseminated candidiasis. Commensal bacteria such as Lactobacillus rhamnosus can antagonize C...
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Article
Immune regulation by fungal strain diversity in inflammatory bowel disease
The fungal microbiota (mycobiota) is an integral part of the complex multikingdom microbial community colonizing the mammalian gastrointestinal tract and has an important role in immune regulation1–6. Although ab...
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Article
Candida pathogens induce protective mitochondria-associated type I interferon signalling and a damage-driven response in vaginal epithelial cells
Vaginal candidiasis is an extremely common disease predominantly caused by four phylogenetically diverse species: Candida albicans; Candida glabrata; Candida parapsilosis; and Candida tropicalis. Using a time cou...
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Protocol
Candida albicans Interaction with Oral Epithelial Cells: Adhesion , Invasion, and Damage Assays
Microbial interactions with epithelial barriers are important steps preceding disease. Infections with Candida albicans are no exception. This opportunistic fungus, commonly harmlessly residing in close proximity...
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Protocol
Experimental Evolution of Candida by Serial Passaging in Host Cells
Experimental evolution is an experiment class of its own; instead of requiring an a priori hypothesis, the genetic adaptation of microbes to defined environments tells us about the underlying pathways and mech...
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Article
Open AccessAntibiotics create a shift from mutualism to competition in human gut communities with a longer-lasting impact on fungi than bacteria
Antibiotic treatment has a well-established detrimental effect on the gut bacterial composition, but effects on the fungal community are less clear. Bacteria in the lumen of the gastrointestinal tract may limi...
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Article
Journal Club
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Article
Open AccessCandidalysin activates innate epithelial immune responses via epidermal growth factor receptor
Candida albicans is a fungal pathobiont, able to cause epithelial cell damage and immune activation. These functions have been attributed to its secreted toxin, candidalysin, though the molecular mechanisms are p...
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Article
CARD9+ microglia promote antifungal immunity via IL-1β- and CXCL1-mediated neutrophil recruitment
The C-type lectin receptor–Syk (spleen tyrosine kinase) adaptor CARD9 facilitates protective antifungal immunity within the central nervous system (CNS), as human deficiency in CARD9 causes susceptibility to f...
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Article
Open AccessThe fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes
Clearance of invading microbes requires phagocytes of the innate immune system. However, successful pathogens have evolved sophisticated strategies to evade immune killing. The opportunistic human fungal patho...
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Article
The needle and the damage done
Bacteria use sophisticated translocation systems to manipulate or kill host cells and competing bacteria. A new study shows that bacteria also deliver anti-fungal effectors.
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Article
Open AccessCorrection: Corrigendum: Dual-species transcriptional profiling during systemic candidiasis reveals organ-specific host-pathogen interactions
Scientific Reports 6: Article number: 36055; published online: 03 November 2016; updated: 19 December 2016
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Article
Open AccessDual-species transcriptional profiling during systemic candidiasis reveals organ-specific host-pathogen interactions
Candida albicans is a common cause of life-threatening fungal bloodstream infections. In the murine model of systemic candidiasis, the kidney is the primary target organ while the fungal load declines over time i...
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Article
Candidalysin is a fungal peptide toxin critical for mucosal infection
Cytolytic proteins and peptide toxins are classical virulence factors of several bacterial pathogens which disrupt epithelial barrier function, damage cells and activate or modulate host immune responses. Such...
