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Article
Open AccessActivation of senescence in critically ill patients: mechanisms, consequences and therapeutic opportunities
Whereas aging is a whole-organism process, senescence is a cell mechanism that can be triggered by several stimuli. There is increasing evidence that critical conditions activate cell senescence programs irres...
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Article
Open AccessCytosolic copper is a major modulator of germination, development and secondary metabolism in Streptomyces coelicolor
Streptomycetes are important biotechnological bacteria with complex differentiation. Copper is a well-known positive regulator of differentiation and antibiotic production. However, the specific mechanisms buf...
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Protocol
Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation
The extension and biological role of Ser/Thr/Tyr phosphorylation in prokaryotes have been only scarcely studied. In this chapter, we describe the state of the art of microbial phosphoproteomics, focusing on pr...
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Article
Open AccessSubcompartmentalization by cross-membranes during early growth of Streptomyces hyphae
Bacteria of the genus Streptomyces are a model system for bacterial multicellularity. Their mycelial life style involves the formation of long multinucleated hyphae during vegetative growth, with occasional cross...
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Article
New ΦBT1 site-specific integrative vectors with neutral phenotype in Streptomyces
Integrative plasmids are one of the best options to introduce genes in low copy and in a stable form into bacteria. The ΦC31-derived plasmids constitute the most common integrative vectors used in Streptomyces. T...
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Article
Open AccessCharacterization of SCO4439, a D-alanyl-D-alanine carboxypeptidase involved in spore cell wall maturation, resistance and germination in Streptomyces coelicolor
This work contributes to the understanding of cell wall modifications during sporulation and germination in Streptomyces by assessing the biological function and biochemical properties of SCO4439, a D-alanyl-D-al...
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Article
Open AccessStreptomyces natalensis programmed cell death and morphological differentiation are dependent on oxidative stress
Streptomyces are aerobic Gram-positive bacteria characterized by a complex life cycle that includes hyphae differentiation and spore formation. Morphological differentiation is triggered by stressful conditions a...
