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Ribosomal Pulldown Assays and Their Use to Analyze Gene Expression in Multiple Inner Ear Cell Types
Research of inner ear cell development and function has greatly benefited from the advent of next-generation sequencing. Cell type-specific analysis of gene expression by RNA sequencing (RNA-seq) has mediated ...
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Article
gEAR: Gene Expression Analysis Resource portal for community-driven, multi-omic data exploration
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Article
Helios is a key transcriptional regulator of outer hair cell maturation
The sensory cells that are responsible for hearing include the cochlear inner hair cells (IHCs) and outer hair cells (OHCs), with the OHCs being necessary for sound sensitivity and tuning1. Both cell types are th...
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Open AccessA comparative analysis of library prep approaches for sequencing low input translatome samples
Cell type-specific ribosome-pulldown has become an increasingly popular method for analysis of gene expression. It allows for expression analysis from intact tissues and monitoring of protein synthesis in vivo...
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Open AccessThe impact of biological sex on the response to noise and otoprotective therapies against acoustic injury in mice
Noise-induced hearing loss (NIHL) is the most prevalent form of acquired hearing loss and affects about 40 million US adults. Among the suggested therapeutics tested in rodents, suberoylanilide hydroxamic acid...
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Open AccessGfi1Cre mice have early onset progressive hearing loss and induce recombination in numerous inner ear non-hair cells
Studies of developmental and functional biology largely rely on conditional expression of genes in a cell type-specific manner. Therefore, the importance of specificity and lack of inherent phenotypes for Cre-dri...
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Open AccessRFX transcription factors are essential for hearing in mice
Sensorineural hearing loss is a common and currently irreversible disorder, because mammalian hair cells (HCs) do not regenerate and current stem cell and gene delivery protocols result only in immature HC-lik...
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Open AccessMap of open and closed chromatin domains in Drosophila genome
Chromatin compactness has been considered a major determinant of gene activity and has been associated with specific chromatin modifications in studies on a few individual genetic loci. At the same time, genom...
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Open AccesshZip2 and hZip3 zinc transporters are down regulated in human prostate adenocarcinomatous glands
The normal human prostate glandular epithelium has the unique function of accumulating high levels of zinc. In prostate cancer this capability is lost as an early event in the development of the malignant cell...
