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Article
Open AccessMolecular signatures of angiogenesis inhibitors: a single-embryo untargeted metabolomics approach in zebrafish
Angiogenesis is a key process in embryonic development, a disruption of this process can lead to severe developmental defects, such as limb malformations. The identification of molecular perturbations represen...
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Article
Open AccessA high-throughput metabolomics in vitro platform for the characterization of hepatotoxicity
Cell-based metabolomics provides multiparametric physiologically relevant readouts that can be highly advantageous for improved, biologically based decision making in early stages of compound development. Here...
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Article
Open AccessApplication of high throughput in vitro metabolomics for hepatotoxicity mode of action characterization and mechanistic-anchored point of departure derivation: a case study with nitrofurantoin
Omics techniques have been increasingly recognized as promising tools for Next Generation Risk Assessment. Targeted metabolomics offer the advantage of providing readily interpretable mechanistic information a...
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Article
3D-Hunde-Lebersphäroide für die präklinische in vitro-Testung
Generation of dog 3D spheroids has been hampered by the fact that dog hepatocytes do not always form spheroids in standard 96-well low attachment plates making in vitro hepatotoxicity testing in high-throughput-f...
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Article
Kofaktor-Mikroarrays zur Identifikation von Kernrezeptorliganden
Different in vitro approaches exist to identify agonistic or antagonistic activity of chemical substances concerning nuclear receptors, since adverse effects in vivo can be the consequence. The recruitment of cor...
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Article
Open AccessIn vitro-to-in vivo extrapolation (IVIVE) by PBTK modeling for animal-free risk assessment approaches of potential endocrine-disrupting compounds
While in vitro testing is used to identify hazards of chemicals, nominal in vitro assay concentrations may misrepresent potential in vivo effects and do not provide dose–response data which can be used for a r...
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Article
Thrombin causes the Enrichment of Rat Brain Primary Cultures with Ependymal Cells Via Protease-Activated Receptor 1
Ependymal cell culture models from rat have been developed over the last 20 years to facilitate biochemical studies on this least-studied glial cell type. The cell culture protocol calls for the presence of th...
