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Article
Ion-Exchange Chromatographic Supports Obtained by Formation of Polyelectrolyte Multi-Layers for the Separation of Proteins
High performance liquid chromatography (HPLC) was used to study the mechanism of formation of polyelectrolyte multilayers on porous silicas. The coatings were produced by alternating the adsorption of positive...
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Article
Coupling of Antibodies to β-Cyclodextrin-Coated Gold Surfaces via an Intermediate Adamantyl-Modified Carboxymethylated Dextran Layer
β-cyclodextrin-coated sensors chips were obtained by grafting amino-β-cyclodextrin or amino-polymers of β-cyclodextrin (poly-β-CD-NH2) to functionalized gold surfaces. An additional carboxymethylated dextran bear...
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Article
Chromatographic properties of chiral stationary phases based on human serum albumin. Influence of the polymer sublayer
Chiral stationary phases based on human serum albumin were prepared by using a reactive polymer, polyacryloyl chloride, to attach the protein to the silica particles. The retention data of the enantiomers depe...
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Chapter and Conference Paper
Original Associating System Between Hydrophobically Modified N,N-Dimethylacrylamide Hydroxyethylmetha-Crylate Copolymers and Water Soluble ß-Cyclodextrins Polymers
Hydrophobically modified polymers or associative polymers (AP) were used as thickeners in food or latex based paints, or as additives in enhanced oil recovery or water treatment [1–5]. They consist in hydrophy...
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Chapter and Conference Paper
The Role of Host - Guest Interaction in Thickening and Phase Separation of B-Cyclodextrin Polymer and Polymers Bearing Cyclic Hydrophobic Side Groups
A large variety of architectures can be obtained from macromolecular assemblies involving inclusion complexes between polymers in aqueous solutions. Mixtures of hydrophically end-capped PEO with ß-cyclodextrin...
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Article
Retention and enantioselectivity properties of β-cyclodextrin polymers and derivatives on porous silica for reverse-phase liquid chromatographic separation of enantiomers
Chiral stationary phases (CSP) based on porous silica coated with β-cyclodextrin polymer and methylated or acetylated derivatives have been prepared. Their enantiomer separation capability was tested and compa...
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Article
New associating polymer systems involving water soluble β-cyclodextrin polymers
Original associating systems have been obtained by mixing hydrophobically end-capped polyethylene oxide and water soluble β-cyclodextrin polymers in aqueous solutions. The hydrophobic ends of the PEO polymers,...
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Chapter and Conference Paper
New Associating Polymer Systems Involving Water Soluble β-Cyclodextrin Polymers
Original associating systems have been obtained by mixing hydrophobically end-capped polyethylene oxide and water soluble β-cyclodextrin polymers in aqueous solutions. The hydrophobic ends of the PEO polymers,...
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Chapter and Conference Paper
Characterization and Structure of Cyclodextrin -Epichlorohydrin Polymers — Effects of Synthesis Parameters
A series of water-soluble polymers were prepared by reacting α- and β-cyclodextrin with epichlorohydrin in NaOH solutions. Depending on the experimental conditions, polymers having very high molecular weight a...
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Chapter
Spectroscopic Characterisation of the Interferon γ and Analogue II Hydro-Organic Solution or Adsorbed on an Hydrophobic Chromatographic Support
In order to explain the weaker chromatographic retention time observed for the human recombinant Interferon γ (IFNγ) compared to its Analogue II (AII), we have studied these proteins in solution in the eluents...
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Article
High performance liquid chromatographic screening methods for revealing the stereoselectivity of reversible interactions between ligand enantiomers and a counterpart substance
The stereoselectivity of the reversible binding interaction between tryptophan enantiomers and various counterpart substances (oligo- and polysaccharides, proteins) has been studied by HPLC. The properties of ...
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Article
Chromatographic and EPR study of poly(vinylimidazole) copper(II) complexes supported on silica
Cu(II) complexes of poly(1-vinylimidazole) immobilized on silica particles have been used as stationary phases for immobilized metal affinity chromatography of some proteins. Chromatographic data are reported ...
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Article
Enantiomer separations with chromatographic supports based on β-cyclodextrin polymers immobilized on porous silica. Role of the polymer structure in separating ability
Two β-cyclodextrin (β-CD)-containing polymers have been prepared either by condensation of β-CD molecules with a bifunctional reagent or by grafting a β-CD derivative on to a linear polymer (polyvinylimidazole...
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Article
Optimisation of fast protein separations on non-porous silica-based strong anion exchangers
The adsorbed coating technology using various vinylpyrrolidone-vinylimidazole copolymer compositions was carried out on 1.7 μm non-porous monodisperse silica. It was shown that the retention properties and the...
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Article
Extraction and characterisation of anti-oestradiol immunoglobulins from human serum by high-performance liquid chromatography
Immunoglobulins have been extracted from human and rabbit serum by size exclusion high performance liquid chromatography. In the case of rabbit immunized against ethinyl-oestradiol and woman having arterial th...
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Article
Synthese und Eigenschaften von anionischen Austauschern einiger Harze vom N-(Dialkylamino-methyl)-acrylamid-Typ