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Article
Open AccessNovel identification and characterisation of Transient receptor potential melastatin 3 ion channels on Natural Killer cells and B lymphocytes: effects on cell signalling in Chronic fatigue syndrome/Myalgic encephalomyelitis patients
Transient receptor potential melastatin 3 (TRPM3) cation channels are ubiquitously expressed by multiple cells and have an important regulatory role in calcium-dependent cell signalling to help maintain cellul...
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Reference Work Entry In depth
Signal Molecules and Calcium
The 28 mammalian members of the superfamily of transient receptor potential (TRP) channels are cation channels, mostly permeable to both monovalent and divalent cations, and can be subdivided into six main sub...
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Article
TRPC1 channels regulate directionality of migrating cells
Cell migration depends on the generation of structural asymmetry and on different steps: protrusion and adhesion at the front and traction and detachment at the rear part of the cell. The activity of Ca2+ channel...
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Chapter
Insights into TRPM4 Function, Regulation and Physiological Role
In the current review we will summarise data from the recent literature describing molecular and functional properties of TRPM4. Together with TRPM5, these channels are up till now the only molecular candidate...
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Chapter
TRPM8
Originally cloned as a prostate-specific protein, TRPM8 is now best known as a cold- and menthol-activated channel implicated in thermosensation. In this chapter we provide a brief review of current knowledge ...
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Article
Influence of temperature on taste perception
Daily experience tells us that temperature has a strong influence on how we taste. Despite the longstanding interest of many specialists in this aspect of taste, we are only starting to understand the molecula...
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Article
Editorial
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Chapter
Structure–function relationship of the TRP channel superfamily
Transient receptor potential (TRP) channels are involved in the perception of a wide range of physical and chemical stimuli, including temperature and osmolarity changes, light, pain, touch, taste and pheromon...
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Chapter
Structure–function relationship of the TRP channel superfamily
Transient receptor potential (TRP) channels are involved in the perception of a wide range of physical and chemical stimuli, including temperature and osmolarity changes, light, pain, touch, taste and pheromon...
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Article
TRPs Make Sense
Abstract
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Article
Block of volume-regulated anion channels by selective serotonin reuptake inhibitors
We have used the whole-cell patch clamp technique to study the effects of the commonly used antidepressants sertraline, paroxetine, citalopram and fluvoxamine on the volume-regulated anion channel (VRAC) in e...
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Article
Ca2+-activated Cl– channels in Ehrlich ascites tumor cells are distinct from mCLCA1, 2 and3
Using the whole-cell patch-clamp technique, we have studied the electrophysiological and pharmacological properties of the Ca2+-activated Cl– current present in Ehrlich cells. The currents activated slowly upon ...
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Article
Extra- and Intracellular Proton-Binding Sites of Volume-Regulated Anion Channels
We have investigated the effects of extracellular and intracellular pH on single channel and macroscopic (macropatches) currents through volume-regulated anion channels (VRAC) in endothelial cells.
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Article
Effect of urea and osmotic cell shrinkage on Ca2+ entry and contraction of vascular smooth muscle cells
The present study was performed to elucidate the effects of urea on vascular smooth muscle cells (SMC). Addition of urea (20, 50, 100 mM) to physiological salt solution blunted the vasoconstrictory effect of p...
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Article
Reduction of ionic strength activates single volume-regulated anion channels (VRAC) in endothelial cells
We have previously shown that a reduction of intracellular ionic strength is involved in the activation of volume-regulated anion channels (VRAC). Here we show in a single-channel study that VRAC can be activa...
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Article
Interaction between calcium-activated chloride channels and the cystic fibrosis transmembrane conductance regulator
We investigated interactions between cystic fibrosis conductance regulator (CFTR) and endogenous Ca2+-activated Cl– channels (CaCC) in bovine pulmonary artery endothelium (CPAE). CPAE cells, which do not express...
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Article
Characterisation of explanted endothelial cells from mouse aorta: electrophysiology and Ca2+ signalling
We describe here the isolation and primary culture of endothelial cells from mouse aorta ("primary explant technique"). These cells provide an excellent model for functional studies in transgenic mice. The pr...
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Article
Nonselective Cation Channels in Endothelial Cells Derived from Human Umbilical Vein
(i) We have used a combined patch-clamp and fura-2 fluorescence technique to characterize a nonselective cation channel (NSC) in Ea.hy926 (EA) cells, an endothelial cell line derived from human umbilical vein...
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Article
Modulation of volume-regulated anion channels by extra- and intracellular pH
We have studied the modulation of the volume-regulated anion channel (VRAC) in cultured endothelial cells from bovine pulmonary artery (CPAE cells) by extra- and intracellular pH. The patch-clamp technique wa...
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Article
Separate Swelling- and Ca2+-activated Anion Currents in Ehrlich Ascites Tumor Cells
A Ca2+-activated (I Cl,Ca) and a swelling-activated anion current (I Cl,vol) were investigated in Ehrlich ascites tumor cells using the whole cell patch clamp te...
