-
Article
Open AccessA Case Study from the Overexpression of OsTZF5, Encoding a CCCH Tandem Zinc Finger Protein, in Rice Plants Across Nineteen Yield Trials
Development of transgenic rice overexpressing transcription factors involved in drought response has been previously reported to confer drought tolerance and therefore represents a means of crop improvement. W...
-
Article
Overexpression of the DREB1A gene under stress-inducible promoter delays leaf senescence and improves drought tolerance in rice
Rice (Oryza sativa) is the major source of food as over half of the world's population relies on rice as their major source of nutrition. Drought stress is the main factor negatively affecting rice growth and pro...
-
Article
Open AccessA beginner’s guide into curated analyses of open access datasets for biomarker discovery in neurodegeneration
The discovery of surrogate biomarkers reflecting neuronal dysfunction in neurodegenerative diseases (NDDs) remains an active area of research. To boost these efforts, we demonstrate the utility of publicly ava...
-
Article
Open Accessα-Synuclein pathology in Parkinson disease activates homeostatic NRF2 anti-oxidant response
Circumstantial evidence points to a pathological role of alpha-synuclein (aSyn; gene symbol SNCA), conferred by aSyn misfolding and aggregation, in Parkinson disease (PD) and related synucleinopathies. Several fi...
-
Article
Open AccessTrans-synaptic spreading of alpha-synuclein pathology through sensory afferents leads to sensory nerve degeneration and neuropathic pain
Pain is a common non-motor symptom of Parkinson’s disease (PD), with current limited knowledge of its pathophysiology. Here, we show that peripheral inoculation of mouse alpha-synuclein (α-Syn) pre-formed fibr...
-
Article
A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli
Polymerases are enzymes that synthesize long chains or polymers of nucleic acids including DNA or RNA from nucleotides. They assemble nucleic acids by copying a DNA or RNA template strand using base-pairing in...
-
Article
Introduction of Arabidopsis’s heat shock factor HsfA1d mitigates adverse effects of heat stress on potato (Solanum tuberosum L.) plant
Thermal stress induces a wide array of morphological and physiological changes in potato affecting its development and economic yield. Response to thermal stress in plants is mostly regulated by heat shock fac...
-
Article
Translational control in brain pathologies: biological significance and therapeutic opportunities
Messenger RNA (mRNA) translation is the terminal step in protein synthesis, providing a crucial regulatory checkpoint for this process. Translational control allows specific cell types to respond to rapid chan...
-
Article
Open AccessActivity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity
Parkinson disease (PD) is the second most common neurodegenerative disorder and the leading neurodegenerative cause of motor disability. Pathologic accumulation of aggregated alpha synuclein (AS) protein in br...
-
Article
MYCN amplified neuroblastoma requires the mRNA translation regulator eEF2 kinase to adapt to nutrient deprivation
MYC family proteins are implicated in many human cancers, but their therapeutic targeting has proven challenging. MYCN amplification in childhood neuroblastoma (NB) is associated with aggressive disease and high ...
-
Article
eEF2K inhibition blocks Aβ42 neurotoxicity by promoting an NRF2 antioxidant response
Soluble oligomers of amyloid-β (Aβ) impair synaptic plasticity, perturb neuronal energy homeostasis, and are implicated in Alzheimer’s disease (AD) pathogenesis. Therefore, significant efforts in AD drug disco...
-
Chapter
Investigation of the Use of Solar Thermal Buffer Zone in Buildings
This paper presents results of a collaborative study that is being carried out by the Thermal Processing Laboratory (TPL), the Department of Civil Engineering and Public Works and Government Services Canada (P...
-
Article
Comparative functional analysis of six drought-responsive promoters in transgenic rice
Rice production is greatly affected by environmental stresses such as drought and high salinity. Transgenic rice plants tolerant to such stresses are expected to be produced. Stress-responsive promoters with l...
-
Protocol
Establishing the Links Between Aβ Aggregation and Cytotoxicity In Vitro Using Biophysical Approaches
Aggregation and fibril formation of the amyloid-β (Aβ) peptides play a pivotal role in the pathogenesis of Alzheimer’s disease (AD). The missing links on the pathway to Aβ oligomerization, fibril formation, an...
-
Article
Preparation and characterization of toxic Aβ aggregates for structural and functional studies in Alzheimer's disease research
The amyloid cascade hypothesis, supported by strong evidence from genetics, pathology and studies using animal models, implicates amyloid-β (Aβ) oligomerization and fibrillogenesis as central causative events ...
-
Article
Characterization of a histidine- and alanine-rich protein showing interaction with calreticulin in rice
Calreticulin (CRT) is a major calcium-sequestering protein in the endoplasmic reticulum and has been implicated in a variety of cellular functions. To analyze the function of CRT in rice, a yeast two-hybrid pr...
-
Article
The rice OsGAE1 is a novel gibberellin-regulated gene and involved in rice growth
Gibberellins (GAs) are a class of phytohormones that regulate many aspects of plant growth and development processes including stem elongation, flowering, and seed germination. A novel GA-enhanced gene, design...
-
Article
Identification of phosphoproteins regulated by gibberellin in rice leaf sheath
To identify the gibberellin (GA) signaling components involved in rice leaf sheath elongation process, protein phosphorylation changed by GA3 was analyzed. The protein kinase activities in rice leaf sheath were a...
