Page
%P
-
Article
A protocol for differential display of mRNA expression using either fluorescent or radioactive labeling
Since its invention in the early 1990s, differential display (DD) has become one of the most commonly used techniques for identifying differentially expressed genes at the mRNA level. Unlike other genomic appr...
-
Book
-
Article
Analysing differential gene expression in cancer
Analysis of messenger RNA and proteins is widely used to compare patterns of gene expression between cells or tissues of different kinds and under different conditions; for example, between normal and cancer c...
-
Protocol
Sensitive Assays for Detection of Lung Cancer
The value of early detection is clear from current tests for specific cancers: self-examination and mammography for breast, prostate-specific antigen (PSA) for prostate, and Pap smears for cervical cancers. No...
-
Article
Conceptual biology: Unearthing the gems
The conceptual review should take its place as an essential component of scientific research.
-
Chapter and Conference Paper
Expression Genetics of Hormone Dependent Human Tumors
Proliferation of some cells in organs such as prostate, breast, and ovary is stimulated by hormones. The hormones function by binding to their receptors, thereby activating them to form complexes with other pr...
-
Article
A Therapeutic Target for Hormone-independent Estrogen Receptor-positive Breast Cancers
The action of the steroid hormone estradiol (E2) is mediated via interaction with a specific receptor (ER) that initiates a series of events downstream, leading to the modulation of hormone-responsive genes an...
-
Protocol
Solid Tumor Cancer Markers and Applications to Steroid Hormone Research
Steroid hormones bind to their specific nuclear receptor protein, which are bound to their DNA receptor motifs and become activated to turn on transcription of other genes (1). As an important example, estrogen b...
-
Article
Potent Induction of Apoptosis by β-Lapachone in Human Multiple Myeloma Cell Lines and Patient Cells
Human multiple myeloma (MM) remains an incurable hematological malignancy. We have reported that β-lapachone, a pure compound derived from a plant, can induce cell death in a variety of human carcinoma cells, inc...
-
Article
Activation of the p21WAF1/CIP1 promoter independent of p53 by the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) through the Sp1 sites
Suberoylanilide hydroxamic acid (SAHA) is a novel histone deacetylase inhibitor with high potency in inducing differentiation of cultured murine erythroleukemia cells. We have recently demonstrated that SAHA i...
-
Article
Suberoylanilide Hydroxamic Acid as a Potential Therapeutic Agent for Human Breast Cancer Treatment
Suberoylanilide hydroxamic acid (SAHA) is a prototype of the newly developed, second-generation, hybrid polar compounds. It is a novel histone deacetylase inhibitor with high potency for inducing cell differen...
-
Article
β-Lapachone Induces Cell Cycle Arrest and Apoptosis in Human Colon Cancer Cells
Human colon cancers have a high frequency of p53 mutations, and cancer cells expressing mutant p53 tend to be resistant to current chemo- and radiation therapy. It is thus important to find therapeutic agents ...
-
Article
A Strategy to Identify Genes Associated with Circulating Solid Tumor Cell Survival in Peripheral Blood
Efforts in metastasis research have centered on the phenotypic and genetic differences between primary site and metastatic site tumors. However, genes that may be used as molecular markers of metastasis in cir...
-
Article
Release of Mitochondrial Cytochrome C in Both Apoptosis and Necrosis Induced by β-Lapachone in Human Carcinoma Cells
There are two fundamental forms of cell death: apoptosis and necrosis. Molecular studies of cell death thus far favor a model in which apoptosis and necrosis share very few molecular regulators. It appears tha...
-
Article
Differential display
Characterization of regulated gene expression in eukaryotic cells is essential for studying cell growth and differentiation as well as for understanding the molecular mechanisms of diseases. Differential displ...
-
Article
Down-Regulation of Laminin-5 in Breast Carcinoma Cells
Laminin-5 (ln-5), a large heterotrimeric glycoprotein consisting of an α3, β3, and γ2 chain, is a component of epithelial cell basement membranes that functions as a ligand of the α3βl and α6β4 integrins to regul...
-
Article
Classification of Breast Cancer Cells on the Basis of a Functional Assay for Estrogen Receptor
The receptor (ER) for estrogen (E2) is routinely assayed as a marker to determine the feasibility of anti-hormone therapy against breast cancer because ER-positive (ER+) tumors are much more likely to respond ...
-
Article
Complete genome expression monitoring: The human race
-
Article
Mob-1, a Ras target gene, is overexpressed in colorectal cancer
Mutations in the ras oncogenes have been linked to many different cancers. In contrast to the extensive body of knowledge related to the genetics of ras activation, relatively little is known of the transcript...
-
Book
