-
Article
Open AccessA Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo
Streptococcus pyogenes Cas9 (SpCas9) and derived enzymes are widely used as genome editors, but their promiscuous nuclease activity often induces undesired mutations and chromosomal rearrangements. Several strate...
-
Article
Open AccessLoss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expression
Genome-wide studies have uncovered multiple independent signals at the RREB1 locus associated with altered type 2 diabetes risk and related glycaemic traits. However, little is known about the function of the zin...
-
Article
Open AccessA genome-wide CRISPR screen identifies CALCOCO2 as a regulator of beta cell function influencing type 2 diabetes risk
Identification of the genes and processes mediating genetic association signals for complex diseases represents a major challenge. As many of the genetic signals for type 2 diabetes (T2D) exert their effects t...
-
Article
Open AccessSystemic gene therapy with thymosin β4 alleviates glomerular injury in mice
Plasma ultrafiltration in the kidney occurs across glomerular capillaries, which are surrounded by epithelial cells called podocytes. Podocytes have a unique shape maintained by a complex cytoskeleton, which b...
