35 Result(s)
within
Annemarie H. Meijer
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Article
Dissimilation curves as a simple method for the characterization of growth of plant cell suspension cultures
A simple non-invasive method for the characterization of growth of a plant cell suspension in a single culture flask is given. The dissimilation of sugars by a cell-culture causes a loss of weight of the conte...
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Article
Isolation of cytochrome P-450 cDNA clones from the higher plant Catharanthus roseus by a PCR strategy
Cytochrome P-450 monooxygenases are membrane-bound enzymes involved in a wide range of biosynthetic pathways in plants. An efficient PCR strategy for isolating cytochrome P-450 cDNA clones from plant cDNA libr...
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Article
Novel members of a family of AT hook-containing DNA-binding proteins from rice are identified through theirin vitro interaction with consensus target sites of plant and animal homeodomain proteins
The AT hook is an AT-rich DNA-binding domain that occurs three times in mammalian high-mobility-group I/Y chromosomal proteins and has recently also been identified in DNA-binding proteins from plants. We unex...
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Article
Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis
For identification of genes involved in embryogenesis in the model cereal rice, we have constructed a collection of cDNA libraries of well-defined stages of embryo development before, during and after organ di...
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Chapter
Yeast as Versatile Tool in Transcription Factor Research
Besides representing an important production organism in the biotechnology industry, the yeast Saccharomyces cerevisiae also constitutes a powerful organism for studying the fundamental processes operative in cel...
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Article
Glucocorticoid-inducible gene expression in rice
We have studied the use of a glucocorticoid receptor-based inducible gene expression system in the monocotyledonous model plant rice (Oryza sativa L.). This system, originally developed by T. Aoyama and N.-H. Ch...
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Article
Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice
Plant homeobox genes of the class 1 knox (knotted1-like) type are involved in the regulation of shoot apical meristem formation and function. Their expression generally occurs either throughout the meristem or sp...
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Article
Characterization of five novel dehydration-responsive homeodomain leucine zipper genes from the resurrection plant Craterostigma plantagineum
Homeodomain leucine zipper (HDZip) genes encode putative transcription factors that are unique to plants. A function in regulating processes that are specific for plants is postulated, such as responses to enviro...
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Article
Different subcellular localization and trafficking properties of KNOX class 1 homeodomain proteins from rice
Genes of the KN1-like homeobox (KNOX) class 1 encode transcription factors involved in shoot apical meristem development and maintenance. We studied the subcellular localization of Green Fluorescent Protein-ta...
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Article
Different subcellular localization and trafficking properties of KNOX class 1 homeodomain proteins from rice
Genes of the KN1-like homeobox (KNOX) class 1 encode transcription factors involved in shoot apical meristem development and maintenance. We studied the subcellular localization of Green Fluorescent Protein-ta...
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Article
EU-OSTID: A Collection of Transposon Insertional Mutants for Functional Genomics in Rice
A collection of 1373 unique flanking sequence tags (FSTs), generated from Ac/Ds and Ac transposon lines for reverse genetics studies, were produced in japonica and indica rice, respectively. The Ds and Ac FSTs to...
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Article
A Homeodomain Leucine Zipper Gene from Craterostigma plantagineum Regulates Abscisic Acid Responsive Gene Expression and Physiological Responses
A subset of homeodomain leucine zipper proteins (HDZip) play a role in regulating adaptation responses including developmental adjustment to environmental cues in plants. Here we report the structural and func...
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Article
A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members
The homeodomain leucine zipper (HD-Zip) genes encode transcription factors that have diverse functions in plant development and have often been implicated in stress adaptation. The HD-Zip genes are the most ab...
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Article
Open AccessZebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine
Addiction is a pathological dysregulation of the brain's reward systems, determined by several complex genetic pathways. The conditioned place preference test provides an evaluation of the effects of drugs in ...
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Protocol
Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions
The yeast one-hybrid system is widely recognized as a valuable and straightforward technique to study interactions between transcription factors and DNA. By means of one-hybrid screens, transcription factors o...
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Article
Open AccessRapid screening of innate immune gene expression in zebrafish using reverse transcription - multiplex ligation-dependent probe amplification
With the zebrafish increasingly being used in immunology and infectious disease research, there is a need for efficient molecular tools to evaluate immune gene expression in this model species. RT-MLPA (revers...
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Article
Open AccessComparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tarda
The zebrafish embryo is an important in vivo model to study the host innate immune response towards microbial infection. In most zebrafish infectious disease models, infection is achieved by micro-injection of ba...
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Chapter and Conference Paper
Spatio-temporal Modeling and Simulation of Mycobacterium Pathogenesis Using Petri Nets
Computational modeling of biological systems is becoming increasingly important in the endeavors to better understand complex biological behavior. It enables researchers to perform computerized simulations usi...
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Article
Function of the HD-Zip I gene Oshox22 in ABA-mediated drought and salt tolerances in rice
Oshox22 belongs to the homeodomain-leucine zipper (HD-Zip) family I of transcription factors, most of which have unknown functions. Here we show that the expression of Oshox22 is strongly induced by salt stress, ...
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Article
Open AccessA zebrafish high throughput screening system used for Staphylococcus epidermidisinfection marker discovery
Staphylococcus epidermidis bacteria are a major cause of biomaterial-associated infections in modern medicine. Yet there is little known about the host responses against this normally innocent bacterium in the co...
