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Article
Open AccessNitric oxide-induced ribosome collision activates ribosomal surveillance mechanisms
Impairment of protein translation can cause stalling and collision of ribosomes and is a signal for the activation of ribosomal surveillance and rescue pathways. Despite clear evidence that ribosome collision ...
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Article
Open AccessFUS regulates a subset of snoRNA expression and modulates the level of rRNA modifications
FUS is a multifunctional protein involved in many aspects of RNA metabolism, including transcription, splicing, translation, miRNA processing, and replication-dependent histone gene expression. In this work, w...
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Article
Regulation of translation by site-specific ribosomal RNA methylation
Ribosomes are complex ribozymes that interpret genetic information by translating messenger RNA (mRNA) into proteins. Natural variation in ribosome composition has been documented in several organisms and can ...
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Article
Open AccessThe long non-coding RNA MIR31HG regulates the senescence associated secretory phenotype
Oncogene-induced senescence provides a barrier against malignant transformation. However, it can also promote cancer through the secretion of a plethora of factors released by senescent cells, called the senes...
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Article
Open AccessSelective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites
The centrosome is the master orchestrator of mitotic spindle formation and chromosome segregation in animal cells. Centrosome abnormalities are frequently observed in cancer, but little is known of their origi...
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Article
A high-throughput screen identifies the long non-coding RNA DRAIC as a regulator of autophagy
Autophagy is a conserved degradation process that occurs in all eukaryotic cells and its dysfunction has been associated with various diseases including cancer. While a number of large-scale attempts have rece...
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Article
Open AccessSNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization
We currently have limited knowledge of the involvement of long non-coding RNAs (lncRNAs) in normal cellular processes and pathologies. Here, we identify and characterize SNHG5 as a stable cytoplasmic lncRNA with ...
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Article
The lncRNA MIR31HG regulates p16INK4A expression to modulate senescence
Oncogene-induced senescence (OIS) can occur in response to oncogenic insults and is considered an important tumour suppressor mechanism. Here we identify the lncRNA MIR31HG as upregulated in OIS and find that kno...
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Article
A non-conserved miRNA regulates lysosomal function and impacts on a human lysosomal storage disorder
Sulfatases are key enzymatic regulators of sulfate homeostasis with several biological functions including degradation of glycosaminoglycans (GAGs) and other macromolecules in lysosomes. In a severe lysosomal ...
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Article
Open AccessmicroRNA-9 targets the long non-coding RNA MALAT1 for degradation in the nucleus
microRNAs regulate the expression of over 60% of protein coding genes by targeting their mRNAs to AGO2-containing complexes in the cytoplasm and promoting their translational inhibition and/or degradation. The...
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Article
Open AccessmicroRNA-146a inhibits G protein-coupled receptor-mediated activation of NF-κB by targeting CARD10 and COPS8 in gastric cancer
Gastric cancer is the second most common cause of cancer-related death in the world. Inflammatory signals originating from gastric cancer cells are important for recruiting inflammatory cells and regulation of...
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Article
Open AccessMicroRNA-143 down-regulates Hexokinase 2 in colon cancer cells
MicroRNAs (miRNAs) are well recognized as gene regulators and have been implicated in the regulation of development as well as human diseases. miR-143 is located at a fragile site on chromosome 5 frequently de...
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Article
Open AccessmiR-449 inhibits cell proliferation and is down-regulated in gastric cancer
Gastric cancer is the fourth most common cancer in the world and the second most prevalent cause of cancer related death. The development of gastric cancer is mainly associated with H. Pylori infection leading to...
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Article
A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites
Insertional mutagens such as viruses and transposons are a useful tool for performing forward genetic screens in mice to discover cancer genes. These screens are most effective when performed using hundreds of...
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Article
Open AccessImpairment of alternative splice sites defining a novel gammaretroviral exon within gagmodifies the oncogenic properties of Akv murine leukemia virus
Mutations of an alternative splice donor site located within the gag region has previously been shown to broaden the pathogenic potential of the T-lymphomagenic gammaretrovirus Moloney murine leukemia virus, whil...
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Article
Genome-wide retroviral insertional tagging of genes involved in cancer in Cdkn2a-deficient mice
We have used large-scale insertional mutagenesis to identify functional landmarks relevant to cancer in the recently completed mouse genome sequence. We infected Cdkn2a−/− mice with Moloney murine leukemia virus ...
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Article
Transcriptional silencing of retroviral vectors
Although retroviral vector systems have been found to efficiently transduce a variety of cell types in vitro, the use of vectors based on murine leukemia virus in preclinical models of somatic gene therapy has...
