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    Protocol

    Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs

    Piwi interacting RNAs (piRNAs) are small (∼25 to ∼30 nucleotide) and are expressed in the germline. piRNAs bind to the Piwi subclade of Argonaute proteins and form the core ribonucleoproteins (RNPs) of piRNPs....

    Yohei Kirino, Anastassios Vourekas, Eugene Khandros… in Argonaute Proteins (2011)