8 Result(s)
within
Anastassios Vourekas
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Protocol
cCLIP-Seq: Retrieval of Chimeric Reads from HITS-CLIP (CLIP-Seq) Libraries
HITS-CLIP (High-Throughput Sequencing after in vivo Crosslinking and Immunoprecipitation, CLIP-Seq) libraries contain fragments of the RNA sequences bound in vivo by an RNA binding protein (RBP). Such fragment...
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Article
Direction of leukocyte polarization and migration by the phosphoinositide-transfer protein TIPE2
The polarization of leukocytes toward chemoattractants is essential for their directed migration. Chen and colleagues show that the phosphoinositide-transfer protein TIPE2 functions as a coordinator of leukocy...
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Article
Sequence-dependent but not sequence-specific piRNA adhesion traps mRNAs to the germ plasm
Maternal mRNAs are tethered within the Drosophila germ plasm via base-pairing interactions between mRNAs and piRNPs containing the Aub Piwi protein; the preference for certain mRNAs to be tethered appears to be r...
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Protocol
HITS-CLIP (CLIP-Seq) for Mouse Piwi Proteins
Piwi proteins, such as Aubergine in Drosophila and Miwi and Mili in mice, form a major subclade of the Argonaute family, which comprise a distinct class of RNA-binding proteins (RBPs) able to bind small RNAs. Sma...
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Article
Open AccessDynamic recruitment of microRNAs to their mRNA targets in the regenerating liver
Validation of physiologic miRNA targets has been met with significant challenges. We employed HITS-CLIP to identify which miRNAs participate in liver regeneration, and to identify their target mRNAs.
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Article
Mili and Miwi target RNA repertoire reveals piRNA biogenesis and function of Miwi in spermiogenesis
Piwi proteins bind a class of small germline RNAs, called piRNAs, whose biogenesis and functions are largely unknown. High-throughput sequencing after cross-linking and immunoprecipitation (HITS-CLIP) analysis...
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Protocol
Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs
Piwi interacting RNAs (piRNAs) are small (∼25 to ∼30 nucleotide) and are expressed in the germline. piRNAs bind to the Piwi subclade of Argonaute proteins and form the core ribonucleoproteins (RNPs) of piRNPs....
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Article
Kinetics of inhibition of ribonuclease P activity by peptidyltransferase inhibitors – Effect of antibiotics on RNase P
A cell-free system derived from Dictyostelium discoideum has been used to study the kinetics of inhibition of RNase P by puromycin, amicetin and blasticidin S. Detailed kinetic analysis showed that the type of in...
