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Protocol
In Vivo Footprinting Analysis in Trichoderma reesei
The in vivo footprinting method identifies protein-targeted DNA regions under different conditions such as carbon sources. Dimethyl sulfate (DMS) generates methylated purine bases at DNA sites which are not bo...
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Article
Open AccessTruncation of the transcriptional repressor protein Cre1 in Trichoderma reesei Rut-C30 turns it into an activator
The filamentous fungus Trichoderma reesei (T. reesei) is a natural producer of cellulolytic and xylanolytic enzymes and is therefore industrially used. Many industries require high amounts of enzymes, in particul...
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Article
Open AccessThe impact of chromatin remodelling on cellulase expression in Trichoderma reesei
Trichoderma reesei is used for industry-scale production of plant cell wall-degrading enzymes, in particular cellulases, but also xylanases. The expression of the encoding genes was so...
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Article
Open AccessXpp1 regulates the expression of xylanases, but not of cellulases in Trichoderma reesei
The ascomycete Trichoderma reesei is industrially used for the production of cellulases. During the production process xylanases are co-secreted, which uses energy and nutrients. Cellulases and xylanases share th...
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Article
Open AccessA truncated form of the Carbon catabolite repressor 1 increases cellulase production in Trichoderma reesei
Rut-C30 is a cellulase-hyperproducing Trichoderma reesei strain and, consequently, became the ancestor of most industry strains used in the production of plant cell wall-degrading enzymes, in particular cellulase...
