24 Result(s)

Article

Effect of the AZOBR_p60123 Plasmid Gene Encoding the Wzt Protein on Lipopolysaccharide Synthesis and Biofilm Formation in the Bacterium Azospirillum baldaniorum Sp245

Inactivation of one of the genes (CDS AZOBR_p60123) of the ABC transporter ATP-binding protein Wzt in the p60123::aphAI mutant of Azospirillum baldaniorum Sp245 and the introduction of an additional copy of this ...

L. P. Petrova, S. S. Evstigneeva, Y. A. Filip’echeva, I. V. Volokhina… in Microbiology (2023)

Article

Resistance of Biofilms Formed by the Soil Bacterium Azospirillum brasilense to Osmotic Stress

Polyethylene glycol (PEG 6000) was used to establish osmotic stress conditions during growth of the type strain Azospirillum brasilense Sp7 and its spontaneous variants Sp7.4 and Sp7.8, because it causes a stable...

D. I. Mokeev, I. V. Volokhina, E. M. Telesheva, S. S. Evstigneeva… in Microbiology (2022)

Article

Genetic Aspects of Mechanosensitivity in the Alphaproteobacteria Azospirillum baldaniorum with Mixed Flagellation

The aim of this work was to test the hypothesis that a putative multisensory hybrid histidine kinase—response regulator (HSHK–RR) and FlhB (a component of the type 3 secretion apparatus located in the basal bo...

S. S. Evstigneeva, D. I. Mokeev… in Molecular Genetics, Microbiology and Virol… (2022)

Article

Variations in Lipopolysaccharide Synthesis Affect Formation of Azospirillum baldaniorum Biofilms in planta at Elevated Copper Content

At an elevated copper content, the viability of Azospirillum baldaniorum on wheat seedling roots and the number of cells colonizing the roots and forming biofilms depended on the physicochemical properties of the...

L. P. Petrova, Yu. A. Filip’echeva, E. M. Telesheva, T. E. Pylaev… in Microbiology (2021)

Article

Suppressed Biofilm Formation Efficiency and Decreased Biofilm Resistance to Oxidative Stress and Drying in an Azospirillum brasilense ahpC Mutant

Azospirillum brasilense forms biofilms on various surfaces. It was previously shown that inactivation of the ahpC gene of alkyl hydroperoxide reductase in the SK586 mutant of A. brasilense Sp245 led to an increas...

A. V. Shelud’ko, D. I. Mokeev, S. S. Evstigneeva, Yu. A. Filip’echeva… in Microbiology (2021)

Article

Cell Ultrastructure in Azospirillum brasilense Biofilms

Due to the primary localization of both epiphytic and endophytic plant growth-promoting rhizobacteria on the surface of the plant root system, biofilm formation is an adaptive trait for these microorganisms. U...

A. V. Shelud’ko, D. I. Mokeev, S. S. Evstigneeva, Yu. A. Filip’echeva… in Microbiology (2020)

Article

Characterization of Carbohydrate-Containing Components of Azospirillum brasilense Sp245 Biofilms

The structure and functions of the biopolymers of the matrix of Azospirillum brasilense biofilms formed at the liquid–solid interface were studied. Calcofluor-binding polysaccharides (CBPS) and the complexes cont...

A. V. Shelud’ko, Yu. A. Filip’echeva, E. M. Telesheva, A. M. Burov… in Microbiology (2018)

Article

Phenol Oxidase Activity of Azospirillum brasilense Sp245 Mutants with Modified Motility and Azospirillum brasilense Sp7 Phase Variants with Different Plasmid Composition

Herein, we reveal the alteration in phenol oxidase enzymes complex production from Azospirillum brasilense Sp245 omegon mutants with polar and lateral flagella dysfunction and from A. brasilense Sp7 phase variant...

E. G. Ponomareva, M. A. Kupryashina, A. V. Shelud’ko, L. P. Petrova… in Microbiology (2018)

Article

Changes in cell surface properties and biofilm formation efficiency in Azospirillum brasilense Sp245 mutants in the putative genes of lipid metabolism mmsB1 and fabG1

The previously obtained insertion mutants of Azospirillum brasilense Sp245 in the genes mmsB1 and fabG1 (strains SK039 and Sp245.1610, respectively) were characterized by impaired flagellation and motility. The p...

E. M. Shumilova, A. V. Shelud’ko, Yu. A. Filip’echeva, S. S. Evstigneeva… in Microbiology (2016)

Article

Changes in biofilm formation in the nonflagellated flhB1 mutant of Azospirillum brasilense Sp245

Bacteria Azospirillum brasilense Sp245 with mixed flagellation are able to form biofilms on various surfaces. A nonflagellated mutant of this strain with inactivated chromosomal copy of the flhB gene (flhB1) was ...

A. V. Shelud’ko, Yu. A. Filip’echeva, E. M. Shumilova, B. N. Khlebtsov… in Microbiology (2015)

Article

Serological relationships of azospirilla revealed by their motility patterns in the presence of antibodies to lipopolysaccharides

Motility of the serologically different Azospirillum brasilense strains Sp245 (serogroup I) and Sp7 (serogroup II) was studied in the presence of antibodies to their lipopolysaccharides (LPS). A procedure was pro...

A. V. Shelud’ko, G. L. Burygin, Yu. A. Filip’echeva, A. E. Belyakov… in Microbiology (2014)

Article

Mutants of bacterium Azospirillum brasilense Sp245 with Omegon insertion in mmsB or fabG genes of lipid metabolism are defective in motility and flagellation

Bacteria Azospirillum brasilense have mixed flagellation: in addition to the polar flagellum, numerous lateral flagella are formed on their cells on medium with increased density. Flagella determine the active sw...

E. A. Kovtunov, A. V. Shelud’ko, M. P. Chernyshova… in Russian Journal of Genetics (2013)

Article

Transposon insertion into a chromosomal copy of flhB gene is concurrent with defects in the formation of polar and lateral flagella in the bacterium Azospirillum brasilense Sp245

Based on an example of Azospirillum brasilense Sp245, it was shown that, in bacteria with mixed flagellation, insertional mutagenesis of one of the copies of the flhB gene, which encodes a component of the flagel...

E. A. Kovtunov, L. P. Petrova, A. V. Shelud’ko, E. I. Katsy in Russian Journal of Genetics (2013)

Article

Negative effect of alkylresorcinols on motility of rhizobacteria Azospirillum brasilense

In liquid media, rhizobacteria Azospirillum brasilense are motile by means of a polar flagellum and in semisolid media, also by means of additionally synthesized lateral flagella. In the presence of 4-n-hexylre-s...

A. V. Shelud’ko, A. V. Tugarova, A. V. Il’chukova, O. E. Varshalomidze… in Microbiology (2013)

Article

Alterations in the primary structure of an 85-MDa plasmid affecting flagellation and motility in the bacterium Azospirillum brasilense Sp245

Earlier, such Azospirillum brasilense Sp245 mutants as flagellation-defective SK051, SK248 with paralyzed flagella, and BK570 swimming and swarming faster than Sp245 were obtained. In SK051 and SK248, the suicide...

E. A. Kovtunov, A. V. Shelud’ko, E. I. Katsy in Russian Journal of Genetics (2012)

Article

Wheat root colonization by Azospirillum brasilense strains with different motility

Migration of associative bacteria Azospirillum brasilense in semisolid media is performed mainly by swarming (Swa⁺ phenotype), which depends on the flagellar functioning and intercellular contacts. Non-swarming m...

A. V. Shelud’ko, A. A. Shirokov, M. K. Sokolova, O. I. Sokolov… in Microbiology (2010)

Article

Localization of denitrification genes in plasmid DNA of bacteria Azospirillum brasilense

In an 85-MDa plasmid (p85) of the plant-associated bacteria Azospirillum brasilense Sp245 the genes encoding copper-containing nitrite reductase (nirK); heterodimeric NO-reductase (norCB); NorQ and NorD proteins ...

L. P. Petrova, O. E. Varshalomidze, A. V. Shelud’ko… in Russian Journal of Genetics (2010)

Article

Plasmid rearrangements and alterations in Azospirillum brasilense biofilm formation

L. P. Petrova, A. V. Shelud’ko, E. I. Katsy in Microbiology (2010)