33 Result(s)

Article

An anchored linkage map for sugar beet based on AFLP, SNP and RAPD markers and QTL map** of a new source of resistance to Beet necrotic yellow vein virus

Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), is an important sugar-beet disease worldwide and can result in severe losses of root yield and sugar content. We have identified a major QTL for BNYV...

M. K. Grimmer, S. Trybush, S. Hanley, S. A. Francis… in Theoretical and Applied Genetics (2007)

Article

Alignment of a Salix linkage map to the Populus genomic sequence reveals macrosynteny between willow and poplar genomes

Poplars (genus Populus) and willows (genus Salix) are members of the Salicaceae, a family of catkin-bearing trees, shrubs and sub-shrubs. Poplar is considered the model system for biological studies in trees and ...

S. J. Hanley, M. D. Mallott, A. Karp in Tree Genetics & Genomes (2006)

Article

A genetic linkage map of willow (Salix viminalis) based on AFLP and microsatellite markers

The genus Salix (willow) contains a number of species of great value as biomass crops. Efforts to breed varieties with improved biomass yields and resistances to pests and diseases are limited by the lack of know...

S. Hanley, J. Barker, J. Van Ooijen, C. Aldam… in Theoretical and Applied Genetics (2002)

Article

A high degree of genetic diversity is revealed in Isatis spp. (dyer's woad) by amplified fragment length polymorphism (AFLP)

Genetic diversity in 38 genotypes, representing 28 individual genotypes from five landraces of Isatis tinctoria (three German: Tubingen, Potsdam and Erfurt, one Swiss and one English), five genotypes of Isatis in...

K. Gilbert (nee Stoker), S. Garton, M. Karam, G. Arnold… in Theoretical and Applied Genetics (2002)

Article

Variation in oil palm (Elaeis guineensis Jacq.) tissue culture-derived regenerants revealed by AFLPs with methylation-sensitive enzymes

Tissue culture-derived plants of oil palm (Elaeis guineensis Jacq.) can develop abnormal flowers in which stamen primordia are converted into carpel-like tissues (mantled fruit). This abnormality can be heritable...

M. Matthes, R. Singh, S.-C. Cheah, A. Karp in Theoretical and Applied Genetics (2001)

Article

An analysis of genetic diversity in coconut (Cocos nucifera) populations from across the geographic range using sequence-tagged microsatellites (SSRs) and AFLPs

Genetic diversity in 31 individuals from 14 coconut populations across the entire geographic range (2–3 individuals per population) was assessed using sequence-tagged microsatellites (or simple sequence repeat...

B. Teulat, C. Aldam, R. Trehin, P. Lebrun… in Theoretical and Applied Genetics (2000)

Article

Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories

A number of PCR-based techniques can be used to detect polymorphisms in plants. For their wide-scale usage in germplasm characterisation and breeding it is important that these marker technologies can be excha...

C.J. Jones, K.J. Edwards, S. Castaglione, M.O. Winfield, F. Sala… in Molecular Breeding (1997)

Article

A comparative study of the mitochondrial genome organization in in vitro cultures of diploid, tetraploid, and hexaploid Triticum species

Southern-blot hybridizations of total DNA to mitochondrial DNA (mtDNA) probes were used to investigate the extent of mtDNA variability in cultures derived from immature embryos of diploid (Triticum monococcum, ge...

C. Hartmann, M. Winfield, F. Corre, M. R. Davey… in Theoretical and Applied Genetics (1996)

Article

Atomic force microscopy of plant chromosomes

Atomic force microscopy has been used to image plant chromosomes from standard preparations without staining or coating. This has enabled the collection of high-resolution three-dimensional data on surface str...

M. Winfield, T. J. McMaster, A. Karp, M. J. Miles in Chromosome Research (1995)

Article

Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes

Mitochondrial (mt) DNAs of somatic hybrids obtained by electrical and chemical fusion of mesophyll protoplasts of S. brevidens and a dihaploid line of S. tuberosum PDH 40 were analysed by Southern hybridization u...

Y. S. Xu, M. G. K. Jones, A. Karp, E. Pehu in Theoretical and Applied Genetics (1993)

Article

Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum

To aid in the identification and analysis of somatic hybrids between potato (Solanum tuberosum, dihaploid line PDH 40) and the non tuber-bearing wild species S. brevidens, a series of species-specific repetitive ...

E. Pehu, M. Thomas, T. Poutala, A. Karp, M. G. K. Jones in Theoretical and Applied Genetics (1990)

Article

Molecular, cytogenetic and morphological characterization of somatic hybrids of dihaploid Solanum tuberosum and diploid S. brevidens

Fifty-eight somatic hybrid plants, produced both by chemical (11) and electrical fusion (47) of protoplasts of dihaploid Solanum tuberosum and S. brevidens, have been analysed by molecular, cytological and morpho...

E. Pehu, A. Karp, K. Moore, S. Steele, R. Dunckley… in Theoretical and Applied Genetics (1989)

Article

Variability in potato tissue culture

Tissue culture systems in potato (Solanum tuberosum L.) that involve a callus phase, such as regeneration of plants from protoplasts or expiants, can be accompanied by phenotypic variability, or somaclonal variat...

A. Karp, M. G. K. Jones, D. Foulger, N. Fish, S. W. J. Bright in American Potato Journal (1989)

Article

Production of somatic hybrids by electrofusion in Solanum

Conditions are described for large scale electrofusion of mesophyll protoplasts of dihaploid S. tuberosum with those of diploid S. brevidens. Overall fusion frequencies of 20%–30% were achieved, and following fus...

N. Fish, A. Karp, M. G. K. Jones in Theoretical and Applied Genetics (1988)

Article

Plant regeneration and somaclonal variation from cultured immature embryos of sister lines of rye and triticale differing in their content of heterochromatin

Plants were regenerated from cultured immature embryos of two pairs of sister lines of triticale (X Triticosecale) cvs Rosner and Drira and five sister lines of rye (Secale cereale). The triticale lines differ in...

P. Bebeli, A. Karp, P. J. Kaltsikes in Theoretical and Applied Genetics (1988)

Article

Isolation, culture and morphogenesis from wheat protoplasts, and study of expression of DNA constructs by direct gene transfer

B. T. Lee, K. Murdoch, J. Top**, M. T. J. de Both… in Plant Cell, Tissue and Organ Culture (1988)

Chapter

Isolation, Culture and Morphogenesis from Wheat Protoplasts, and Study of Expression of DNA Constructs by Direct Gene Transfer

The regeneration of plants from protoplasts of some monocots has been achieved, but so far the list excludes wheat. We report here on the culture of protoplasts obtained from an inflorescence-derived suspensio...

B. T. Lee, K. Murdoch, J. Top**… in Progress in Plant Protoplast Research (1988)

Article

Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium

Derivatives of potato (Solanum tuberosum cv.'s ‘Maris Bard’ and ‘Desiree’) transformed with disarmed T-DNA from genetically engineered Agrobacterium tumefaciens strains were isolated. The transformed plants were ...

G. Ooms, M. M. Burrell, A. Karp, M. Bevan, J. Hille in Theoretical and Applied Genetics (1987)