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Article
Open AccessGenetic diversity, population structure and phenotypic variation in European Salix viminalis L. (Salicaceae)
To investigate the potential of association genetics for willow breeding, Salix viminalis germplasm was assembled from UK and Swedish collections (comprising accessions from several European countries) and new sa...
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Article
An anchored linkage map for sugar beet based on AFLP, SNP and RAPD markers and QTL map** of a new source of resistance to Beet necrotic yellow vein virus
Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), is an important sugar-beet disease worldwide and can result in severe losses of root yield and sugar content. We have identified a major QTL for BNYV...
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Article
Alignment of a Salix linkage map to the Populus genomic sequence reveals macrosynteny between willow and poplar genomes
Poplars (genus Populus) and willows (genus Salix) are members of the Salicaceae, a family of catkin-bearing trees, shrubs and sub-shrubs. Poplar is considered the model system for biological studies in trees and ...
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Article
A genetic linkage map of willow (Salix viminalis) based on AFLP and microsatellite markers
The genus Salix (willow) contains a number of species of great value as biomass crops. Efforts to breed varieties with improved biomass yields and resistances to pests and diseases are limited by the lack of know...
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Article
A high degree of genetic diversity is revealed in Isatis spp. (dyer's woad) by amplified fragment length polymorphism (AFLP)
Genetic diversity in 38 genotypes, representing 28 individual genotypes from five landraces of Isatis tinctoria (three German: Tubingen, Potsdam and Erfurt, one Swiss and one English), five genotypes of Isatis in...
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Article
Variation in oil palm (Elaeis guineensis Jacq.) tissue culture-derived regenerants revealed by AFLPs with methylation-sensitive enzymes
Tissue culture-derived plants of oil palm (Elaeis guineensis Jacq.) can develop abnormal flowers in which stamen primordia are converted into carpel-like tissues (mantled fruit). This abnormality can be heritable...
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Article
An analysis of genetic diversity in coconut (Cocos nucifera) populations from across the geographic range using sequence-tagged microsatellites (SSRs) and AFLPs
Genetic diversity in 31 individuals from 14 coconut populations across the entire geographic range (2–3 individuals per population) was assessed using sequence-tagged microsatellites (or simple sequence repeat...
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Chapter
The use of polymorphic microsatellites for assessing genetic diversity in coconut
Microsatellites, or Simple Sequence Repeats (SSRs) are short tandemly repeated sequence motifs of circa 1–8 bp in length, which are scattered in abundance throughout the genome and can vary between individuals in...
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Chapter
Reproducibility Testing of RAPDs by a Network of European Laboratories
For the application of molecular techniques to wide-scale diversity screening it is important that the different screening techniques employed can be standardized to yield reproducible results across laborator...
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Chapter
Reproducibility Testing of AFLPs by a Network of European Laboratories
The use of molecular techniques in diversity studies by networks of laboratories, where common databases are continually fed with data from all the participating groups, requires that the techniques are reprod...
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Chapter
Reproducibility Testing of SSRs by a Network of European Laboratories
Microsatellites or simple sequence repeats (SSRs) are highly mutable loci which are present at many sites in a genome (see Box 11.1, Chapters 11.1 and 11.2). Since the flanking sequences at each of these sites...
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Article
Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories
A number of PCR-based techniques can be used to detect polymorphisms in plants. For their wide-scale usage in germplasm characterisation and breeding it is important that these marker technologies can be excha...
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Article
Book review
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Article
A comparative study of the mitochondrial genome organization in in vitro cultures of diploid, tetraploid, and hexaploid Triticum species
Southern-blot hybridizations of total DNA to mitochondrial DNA (mtDNA) probes were used to investigate the extent of mtDNA variability in cultures derived from immature embryos of diploid (Triticum monococcum, ge...
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Article
Atomic force microscopy of plant chromosomes
Atomic force microscopy has been used to image plant chromosomes from standard preparations without staining or coating. This has enabled the collection of high-resolution three-dimensional data on surface str...
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Article
Effect of genotype on chromosome variation in tissue culture of inbred and outbred rye
To examine the effect of genotype on the nature of the cytological variation induced in culture, plants were regenerated from cultured immature embryos of six genotypes (lines A-F) of rye (Secale cereale L.). The...
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Article
Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes
Mitochondrial (mt) DNAs of somatic hybrids obtained by electrical and chemical fusion of mesophyll protoplasts of S. brevidens and a dihaploid line of S. tuberosum PDH 40 were analysed by Southern hybridization u...
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Article
Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum
To aid in the identification and analysis of somatic hybrids between potato (Solanum tuberosum, dihaploid line PDH 40) and the non tuber-bearing wild species S. brevidens, a series of species-specific repetitive ...
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Chapter
Chromosome Instability in Bread Wheat (Triticum aestivum) Cell Suspensions and their Dividing Protoplasts
The development of efficient regeneration systems in the cereals is a key factor in the establishment of techniques aimed at direct manipulation of the cereal genome (Jones 1985). Regeneration has been achieve...
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Chapter
Transfer of Resistance to PLRV, PVX and PVY from S.brevidens to Potato by Somatic Hybridisation: Characterisation and Field Evaluation
To understand and apply the technology of protoplast fusion, it is useful to study populations of somatic hybrid plants produced rather than just those hybrids which have the desired properties. This enables a...
