52 Result(s)
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**wen Su
Life Sciences
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Article
Production of rosmarinic acid in high density perfusion cultures ofAnchusa officinalis using a high sugar medium
The most direct approach to enhancing the volumetric yield of secondary metabolites in plant tissue cultures is to operate the culture under high cell density. In this study, a cell suspension ofAnchusa officinal...
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Production of rosmarinic acid from perfusion culture ofAnchusa officinalis in a membrane-aerated bioreactor
In this study, a perfusion fermentation ofAnchusa officinalis was carried out in a stirred tank bioreactor integrated with an internal cross-flow filter. Bubble-free aeration via microporous membrane fibers was u...
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Rosmarinic acid production in perfused Anchusa officinalis culture: Effect of inoculum size
High cell density and rosmarinic acid (RA) productivity have been achieved by applying periodic culture perfusion to the Anchusa officinalis cell suspension. In this study, the effect of inoculum size on cell gro...
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Induction of somatic embryogenesis in Azadirachta indica
A modified culture protocol has been developed for the induction of somatic embryogenesis in Azadirachta indica (neem). Embryogenic calluses were initiated from cotyledons or hypocotyls using a Murashige and Skoo...
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Secreted Metabolite Production in Perfusion Plant Cell Cultures
Operation of a novel bioreactor that allows continuous perfusion cultivation of plant cell suspensions is described in this paper. This external-loop, air-lift bioreactor has an internal settling zone for cell...
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Open AccessThe short coiled-coil domain-containing protein UNC-69 cooperates with UNC-76 to regulate axonal outgrowth and normal presynaptic organization in Caenorhabditis elegans
The nematode Caenorhabditis elegans has been used extensively to identify the genetic requirements for proper nervous system development and function. Key to this process is the direction of vesicles to the growi...
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Open AccessTranscriptome analysis of Deinagkistrodon acutus venomous gland focusing on cellular structure and functional aspects using expressed sequence tags
The snake venom gland is a specialized organ, which synthesizes and secretes the complex and abundant toxin proteins. Though gene expression in the snake venom gland has been extensively studied, the focus has...
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Recombinant production of fibrinogenase IV from Agkistrodon acutus venom and its preliminary evaluation
A novel metalloproteinase, recombinant fibrinogenase IV (rFIVa), was expressed and purified from Agkistrodon acutus venom. It is a single-chain protein with an apparent molecular weight of 27 kDa. Western blot sh...
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Open AccessToo much data, but little inter-changeability: a lesson learned from mining public data on tissue specificity of gene expression
The tissue expression pattern of a gene often provides an important clue to its potential role in a biological process. A vast amount of gene expression data have been and are being accumulated in public repos...
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Article
PaCDPK1, a gene encoding calcium-dependent protein kinase from orchid, Phalaenopsis amabilis, is induced by cold, wounding, and pathogen challenge
Signaling pathways, specifically calcium and calcium-dependent protein kinase (CDPK), have been implicated in the regulation of stress and developmental signals in plants. Here, we reported the isolation and c...
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Bioreactor Engineering For Recombinant Protein Production Using Plant Cell Suspension Culture
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Open AccessTargeted suppression of heme oxygenase-1 by small interference RNAs inhibits the production of bilirubin in neonatal rat with hyperbilirubinemia
Excessive accumulation of bilirubin contributes to neonatal hyperbilirubinemia in rats. Heme oxygenase (HO) is one of the rate-limiting enzymes in catabolizing heme to bilirubin. In the present study, we inves...
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Staged-probability strategy of processing shotgun proteomic data to discover more functionally important proteins
Biologically important proteins related to membrane receptors, signal transduction, regulation, transcription, and translation are usually low in abundance and identified with low probability in mass spectrosc...
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Article
1-Methylcyclopropene prevents cotton physiological and molecular responses to ethylene
The phytohormone ethylene can cause adverse effects in plants, including inhibition of shoot elongation and abscission of leaves, flowers and fruits. 1-Methylcyclopropene (1-MCP) is a competitive inhibitor of ...
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Article
Cloning of Six ERF Family Transcription Factor Genes from Peanut and Analysis of their Expression during Abiotic Stress
Several ethylene-responsive element binding factor (ERF) family proteins have been demonstrated to play important roles in transcriptional regulation of a variety of biological processes, including responses t...
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Inhibition of Camellia sinensis (L.) O. Kuntze on Microcystis aeruginosa and isolation of the inhibition factors
Low concentration of tea (Camellia sinensis (L.) O. Kuntze) was shown to inhibit the growth of the toxic cyanobacterium Microcystis aeruginosa. The inhibition efficiency was 40 % at 0.1 g dry tea/L and 90 % at 0....
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Isolation and Characterization of a Novel Antithrombotic Peptide from Enzymatic Hydrolysate of Agkistrodon acutus Venom
Snake venoms contain a large variety of proteins and peptides that affect the hemostasis and thrombosis. Numerous antithrombotic peptides have been found in snake venom. However, few studies have been performe...
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Article
Estimation of gases emitted by forest fires based on remote sensing data
Forest fire, an important agent for change in many forest ecosystems, plays an important role in atmospheric chemical cycles and the carbon cycle. The primary emissions from forest fire, CO2, CO, CH4, long-chaine...
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Open AccessInteractive effects of C-reactive protein levels on the association between APOE variants and triglyceride levels in a Taiwanese population
Apolipoprotein E (APOE) plays a major role in lipid metabolism and inflammation. However, the association between APOE gene polymorphisms and serum triglyceride levels remains controversial. We tested the effects...
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Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of peanut (Arachis hypogaea L.)
The cultivated peanut is important oil crop and salt stress seriously influences its development and yield. Tolerant varieties produced using transgenic techniques can effectively increase peanut plantation ar...
