6,845 Result(s)
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Production and characterization of antibodies cross-reactive with major aflatoxins
Antibodies cross-reactive with 4 major aflatoxins were demonstrated three weeks after immunization of rabbits with an immunogen which was prepared by conjugating aflatoxin B3 to bovine serum albumin. Aflatoxin B3
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The distribution of calmodulin and Ca2+-activated calmodulin in cell cycle of mouse erythroleukemia cells
Cell proliferation is accompanied with changing levels of intracellular caimodulin (CAM) and its activation. Prior data from synchronized cell population could not actually stand for various CaM levels in diff...
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The development of the region between the preexisting and nascent membranes during the first cleavage of Rana amurensis eggs
The region between the preexisting and nascent membranes of a cleaving Rana egg is a dense protrusion region of nearly 40 μm wide at 180° stage. Later, this region differentiate, into an upper part, a strip with ...
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Intracellular levels of calmodulin are increased in transformed cells
By using Hoechst 33342, rabbit anti calmodulin antibody, FITC-labeled goat anti rabbit IgG and SR101 (sulfo rhodamine 101) simultaneously to stain individual normal and transformed cells, the microspectrophoto...
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Immunocytochemical staining with antibodies against protein kinase C and its isozymes in the turtle retina
An LM immunocytochemical study has investigated the patterns of staining in turtle retina with monoclonal antibodies to the α, β and γ isozymes of protein kinase C. The protein kinase C-γ antibody reveals cell...
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Effect of peripheral nerve cut on neuropeptides in dorsal root ganglia and the spinal cord of monkey with special reference to galanin
Using the indirect immunofluorescence method andin situ hybridization, the localization and levels of immunoreactivities and mRNAs for several neuropeptides were studied in lumbar dorsal root ganglia and spinal c...
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Animal mitochondrial DNA polymorphism: a valuable tool for evolutionary studies
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Histochemical study of the pre- and postnatal development of acetylcholinesterase in the rat spinal cord
The distribution of acetylcholinesterase (AChE)-positive structures in the develo** rat spinal cord was studied with AChE-histochemistry. AChE-positive perikarya were first seen on embryonic day 14 (E14) in ...
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Complex behaviours of AB model describing idiotypic network
A simple chemical model of the idiotypic network of immune systems, namely the AB model, has been developed by De Boeret al. The complexity of the system, such as the steady states, periodic oscillations and chao...
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Article
Human immune suppression is inducible by trichosan- thin via CD8 cell-mediated pathway
Trichosanthin(Tk), a polypeptide with 249 amino acid residues isolated and purified from a Chinese medicinal herb, showed the capability of inducing abortion and was able to inhibit tumor growth and HIV replic...
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Cloning, sequencing and analyzing of the heavy chain V region genes of human polyreactive antibodies
The heavy chain variable region genes of 5 human polyreactive mAbs generated in our laboratory have been cloned and sequenced using polymerase chain reaction (PCR) technique. We found that 2 and 3 mAbs utilize...
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Article
EGF receptor-mediated intracellular calcium increase in human hepatoma BEL-7404 cells
Epidermal growth factor (EGF) induced intracellular free calcium ([Ca2+]i) response was studied in fura-2- or fluo-3-loaded human hepatoma cells of BEL-7404 cell line. Single cell [Ca2+]i analysis and [Ca2+]i mea...
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Observation of nuclei reassembled from demembranated Xenopus sperm nuclei and analysis of their lamina components
A cell-free preparation obtained from extracts of activated Xenopus laevis eggs induced chromatin decondensation and nuclear formation from demembranated Xenopus sperm nuclei. Electron microscopy revealed that th...
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Molecular cloning and structural analysis of human norepinephrine transporter gene(NETHG)
A cDNA molecule encoding a major part of the human Norepinephrine transporter(hNET) was synthesized by means of Polymerase Chain Reaction(PCR) technique and used as a probe for selecting the human genomic NET ...
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Laser scanning fluorescence microscopic measurement of the movement of cleaving egg surface of Rana Amurensis
By laser scanning fluorescence microscopy for quantitative measurement of fluorescence intensity changes on egg surface stained with fluorescein isothiocvanate during cleavage furrow extending forward, it was ...
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Tenascin-C expression by neurons and glial cells in the rat spinal cord: Changes during postnatal development and after dorsal root or sciatic nerve injury
We have usedin situ hybridization with a digoxigenin-labelled probe for tenascin-C mRNA and immunocytochemistry with antibodies against tenascin-C, glial fibrillary acidic protein, OX-42 and the 200 kDa neurofila...
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Differential regulationof glutathione S-transferase Yb1 mRNA levels in rat prostate, liver and brain by androgen
Northern blot analysis of glutathione S-transferase (GST) Yb1 mRNA in different tissues of male and female rats revealed that its tissue-specific transcription patterns were highly sex hormone related. Althoug...
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Substance P enhances the proliferation of rat anterior pituitary cells in vitro
The undecapeptide substance P(SP) was shown to be intimately involved in both the structural and functional aspects of the anterior pituitary. Yet, in addition to its influences on hormonal secretion, SP may w...
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Changes of macromolecular organizations in nonjunctional sarcolemmas after cross-innervation— a study of fast-and slow-twitch muscle fibres in rats
The purpose of the present study was to analyse the changes of macromolecular organizations in nonjunctional sarcolemmas of different types of skeletal muscle fibres after cross-innervation. In normal rats the...
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Correlation between inhibition of calcium-dependent apoptosis by cyclosporin A and calcium transportation in HL-60 cells
Both calcium ionophore A23187 and endoplasmic reticulum Ca2+ - ATPase inhibitor thapsigargin (Tg) could increase intracellular free calcium concentration and induce apoptosis in some cell lines. In the present st...
