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Article
Open AccessCoHIT: a one-pot ultrasensitive ERA-CRISPR system for detecting multiple same-site indels
Genetic testing is crucial for precision cancer medicine. However, detecting multiple same-site insertions or deletions (indels) is challenging. Here, we introduce CoHIT (Cas12a-based One-for-all High-speed Is...
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Article
Optimizing multicopy chromosomal integration for stable high-performing strains
The copy number of genes in chromosomes can be modified by chromosomal integration to construct efficient microbial cell factories but the resulting genetic systems are prone to failure or instability from tri...
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Article
Self-powered wireless environmental monitoring system for in-service bridges by gallo** piezoelectric-triboelectric hybridized energy harvester
The energy harvesting technology for the ubiquitous natural wind enables a desirable solution to the issue of distributed sensors in the bridge environmental sensing Internet of Things (IoT) system being restr...
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Article
A bibliographic review of the relationship between ecosystem services and human well-being
Although the relationship between ecosystem services and human well-being (REH) has been widely recognized and has become an important basis for policy-making, a quantitative review of the relationship is stil...
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Article
Open AccessSubstantially reducing global PM2.5-related deaths under SDG3.9 requires better air pollution control and healthcare
The United Nations’ Sustainable Development Goal (SDG) 3.9 calls for a substantial reduction in deaths attributable to PM2.5 pollution (DAPP). However, DAPP projections vary greatly and the likelihood of meeting ...
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Article
Open AccessMaternal fiber-rich diet promotes early-life intestinal development in offspring through milk-derived extracellular vesicles carrying miR-146a-5p
The intestinal development in early life is profoundly influenced by multiple biological components of breast milk, in which milk-derived extracellular vesicles (mEVs) contain a large amount of vertically tran...
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Article
Enlarged DNA unwinding by Nme2Cas9 permits a broadened base editing window beyond the protospacer
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Article
Open AccessBacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases
Prime editing allows precise installation of any single base substitution and small insertions and deletions without requiring homologous recombination or double-strand DNA breaks in eukaryotic cells. However,...
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Chapter and Conference Paper
Why Query Plans Are Different: An Automatic Detection and Inference System
Preventing plan regression has always been a demanding task. SQL tuning advisor, e.g., index advisor, and optimizer testing tool are two common solutions. The former is proposed for database users to avoid a s...
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Article
Open AccessThe sacrificial record in burial pits of the late Shang Dynasty: evidences from the chroma and magnetic properties of the Sanxingdui site, Sichuan, China
The Sanxingdui site (4.4–2.9 ka B.P.) in southwestern China is considered one of the most significant archaeological discoveries of the twentieth century, which contains numerous codes for interpreting the ori...
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Article
Open AccessProRefiner: an entropy-based refining strategy for inverse protein folding with global graph attention
Inverse Protein Folding (IPF) is an important task of protein design, which aims to design sequences compatible with a given backbone structure. Despite the prosperous development of algorithms for this task, ...
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Article
Open AccessDesign of prime-editing guide RNAs with deep transfer learning
Prime editors (PEs) are promising genome-editing tools, but effective optimization of prime-editing guide RNA (pegRNA) design remains a challenge owing to the lack of accurate and broadly applicable approaches...
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Article
Open AccessCRISPR/Cas9-mediated knockout of intracellular molecule SHP-1 enhances tumor-killing ability of CD133-targeted CAR T cells in vitro
CAR T cell therapy has been successfully used in the treatment of hematological malignancies, and the strategy that deletion of inhibitory receptor on the CAR T cell surface, such as PD-1, greatly enhance the ...
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Article
A Cas12a-based fluorescent microfluidic system for rapid on-site human papillomavirus diagnostics
Persistent infection with human papillomavirus (HPV) is the leading cause of cervical cancer, and early diagnosis is crucial for clinical management. However, the easy and rapid on-site diagnostic for HPV geno...
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Article
Suppression of cytokine release syndrome during CAR-T-cell therapy via a subcutaneously injected interleukin-6-adsorbing hydrogel
The infusion of chimaeric antigen receptor (CAR) T cells can trigger the release of life-threatening supraphysiological levels of pro-inflammatory cytokines. However, uncertainty regarding the timing and sever...
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Article
Open AccessThe challenge of population aging for mitigating deaths from PM2.5 air pollution in China
Estimating the health burden of air pollution against the background of population aging is of great significance for achieving the Sustainable Development Goal 3.9 which aims to substantially reduce the death...
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Article
Open AccessDeletion of ARGLU1 causes global defects in alternative splicing in vivo and mouse cortical malformations primarily via apoptosis
Haploinsufficient mutation in arginine and glutamine-rich protein 1 (Arglu1), a newly identified pre-mRNA splicing regulator, may be linked to neural developmental disorders associated with mental retardation and...
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Article
Structure and engineering of miniature Acidibacillus sulfuroxidans Cas12f1
The miniature CRISPR-Cas12f nucleases enable efficient delivery via cargo-size-limited vehicles, thereby showing promise for in vivo therapeutic applications. Acidibacillus sulfuroxidans Cas12f1 (AsCas12f1, 422 a...
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Article
Lattice Boltzmann method with diffusive scaling for thermal flows in porous media
In this work, we propose a lattice Boltzmann method for thermal flows in porous media, and show that the governing equations for thermal flows in porous media can be recovered correctly through the Maxwell ite...
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Article
Relationships between urban expansion and socioenvironmental indicators across multiple scales of watersheds: a case study among watersheds running through China
Understanding the relationships between urban expansion and social/environmental features is fundamental to managing watershed and urban expansion. However, such relationships remain unclear, especially across...
