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Article
Alleviating symptoms of neurodegenerative disorders by astrocyte-specific overexpression of TMEM164 in mice
Neuroinflammatory microglia secrete cytokines to induce neurotoxic reactive astrocytes, which are one of the major causes of neuronal death. However, the intrinsic key regulators underlying neurotoxic reactive...
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Article
Treatment of autosomal recessive hearing loss via in vivo CRISPR/Cas9-mediated optimized homology-directed repair in mice
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Article
Recognition of weld defects from X-ray images based on improved convolutional neural network
When convolutional neural network (CNN) is used for welding defect detection image recognition, the recognition result will be affected by many factors such as human factors, the activation function is sensiti...
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Article
Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice
Therapeutic genome editing requires effective and targeted delivery methods. The delivery of Cas9 mRNA using adeno-associated viruses has led to potent in vivo therapeutic efficacy, but can cause sustained Cas9 e...
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Article
Open AccessACTA1 is inhibited by PAX3-FOXO1 through RhoA-MKL1-SRF signaling pathway and impairs cell proliferation, migration and tumor growth in Alveolar Rhabdomyosarcoma
Alveolar Rhabdomyosarcoma (ARMS) is a pediatric malignant soft tissue tumor with skeletal muscle phenotype. Little work about skeletal muscle proteins in ARMS was reported. PAX3-FOXO1 is a specific fusion gene ge...
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Article
Endogenous promoter-driven sgRNA for monitoring the expression of low-abundance transcripts and lncRNAs
Detection of endogenous signals and precise control of genetic circuits in the natural context are essential to understand biological processes. However, the tools to process endogenous information are limited...
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Article
Base editing-mediated splicing correction therapy for spinal muscular atrophy
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Chapter and Conference Paper
Weld Flaw Recognition with Improved Convolutional Neural Network
The simple linear iterative clustering (SLIC) algorithm and the improved exponential linear unit (ELU) activation function are used to construct CNN model for weld flaw detection image recognition. First, the ...
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Article
Open AccessScreened AAV variants permit efficient transduction access to supporting cells and hair cells
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Article
Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis
Recently developed DNA base editing methods enable the direct generation of desired point mutations in genomic DNA without generating any double-strand breaks1–3, but the issue of off-target edits has limited the...
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Article
P85 regulates neuronal migration through affecting neuronal morphology during mouse corticogenesis
In mammalian develo** embryonic cortex, projection neurons migrate from the ventricular zone to the cortical plate, guided by radial glial cells with a transformation between bipolar and multipolar morpholog...
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Article
In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice
Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of multiple genes remains challenging. We generated a transgenic mouse using an improved dCas9 system that enables simu...
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Article
Abundant Focal Adhesion Kinase Causes Aberrant Neuronal Migration Via Its Phosphorylation at Tyr925
The process of neuronal migration is precisely regulated by different molecules during corticogenesis. The FAK (focal adhesion kinase) plays a critical role in embryogenesis and is involved in cell motility th...
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Chapter
UHVAC Electrical Equipment
The UHVAC electrical equipment, which is the base of the UHVAC project and whose performance directly affects the stability of the UHV line, is the important research content of the UHVAC technology. This chap...
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Article
Open AccessCRISPR/Cas9-mediated targeted chromosome elimination
The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and deletion of chromosomal segments. However, whether an en...
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Article
Open AccessOne-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs
The CRISPR/Cas9 system is an efficient gene-editing method, but the majority of gene-edited animals showed mosaicism, with editing occurring only in a portion of cells. Here we show that single gene or multipl...
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Article
Open AccessHomology-mediated end joining-based targeted integration using CRISPR/Cas9
Targeted integration of transgenes can be achieved by strategies based on homologous recombination (HR), microhomology-mediated end joining (MMEJ) or non-homologous end joining (NHEJ). The more generally used ...
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Article
The function of sperm-associated antigen 6 in neuronal proliferation and differentiation
Sperm-associated antigen 6 (SPAG6) is initially found in human testis and is essential for sperm motility and male fertility. Later studies indicate that it also express in the chick Central Nervous System and...
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Article
Open AccessAdvanced Fabrication of Chemically Bonded Graphene/TiO2 Continuous Fibers with Enhanced Broadband Photocatalytic Properties and Involved Mechanisms Exploration
In this article, a novel route for the synthesis of graphene/TiO2 continuous fibers (GTF) using force-spinning combined with water vapor annealing method is reported for the first time. The morphology, structure ...
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Article
Spag6 Negatively Regulates Neuronal Migration During Mouse Brain Development
Sperm-associated antigen 6 (Spag6) is a Chlamydomonas reinhardtii PF16 homologous gene detected in the human testis and is crucial for sperm motility. Neuronal migration is a dynamic process requiring coordinated...
